Luciferases from BACTERIA such as PHOTOBACTERIUM; VIBRIO; and PHOTORHABDUS.

The family Lampyidae, which are bioluminescent BEETLES. They contain FIREFLY LUCIFERIN and LUCIFERASES. Oxidation of firefly luciferin results in luminescence.

Luciferases from FIREFLIES, usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS.

Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.

A benzothaizole which is oxidized by LUCIFERASES, FIREFLY to cause emission of light (LUMINESCENCE).

A family of gram-negative bacteria whose members predominate in the bacterial flora of PLANKTON; FISHES; and SEAWATER. Some members are important pathogens for humans and animals.

INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.

Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.

Luciferases from RENILLA that oxidizes certain LUMINESCENT AGENTS to cause emission of PHOTONS.

Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.

A genus of bioluminescent marine invertebrates in the family Renillidae, order Pennatulacea, class ANTHOZOA. It contains Renilla LUCIFERASE which oxidizes coelenterazine resulting in LUMINESCENCE.

A huge subclass of mostly marine CRUSTACEA, containing over 14,000 species. The 10 orders comprise both planktonic and benthic organisms, and include both free-living and parasitic forms. Planktonic copepods form the principle link between PHYTOPLANKTON and the higher trophic levels of the marine food chains.

Organisms that live in water.

A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that are common in the marine environment and on the surfaces and in the intestinal contents of marine animals. Some species are bioluminescent and are found as symbionts in specialized luminous organs of fish.

Compound such as LUMINESCENT PROTEINS that cause or emit light (PHYSICAL LUMINESCENCE).

A genus of gram-negative bacteria existing symbiotically with nematodes of the family Heterorhabditidae (see RHABDITOIDEA). These nematodes infect a variety of soil-dwelling insects. Upon entering an insect host, the nematode releases Photorhabdus from its intestinal tract and the bacterium establishes a lethal septicemia in the insect.

The reconstitution of a protein's activity following denaturation.

The visually perceived property of objects created by absorption or reflection of specific wavelengths of light.

The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.

A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.

Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.

Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The rate dynamics in chemical or physical systems.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

Proteins prepared by recombinant DNA technology.

The parts of a macromolecule that directly participate in its specific combination with another molecule.