• Caco-2 cells labeled for tight junction molecule cingulin (green), actin (red), vinculin (pink) and DNA (blue). (weizmann.ac.il)
  • Advanced glycation endproducts interacting with their endothelial receptor induce expression of vascular cell adhesion molecule-1 (VCAM-1) in cultured human endothelial cells and in mice. (jci.org)
  • Vascular cell adhesion molecule-1 (VCAM-1), an inducible cell-cell recognition protein on the endothelial cell surface (EC), has been associated with early stages of atherosclerosis. (jci.org)
  • Human Neural cell adhesion molecule 1 (NCAM1) ELISA kit is Available at Gentaur Genprice with the fastest delivery. (joplink.net)
  • We show here that the interactions between DV E protein, the sole mannosylated glycoprotein present on DV particles, and the C-type lectin dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) are essential for DV infection of DCs. (unboundmedicine.com)
  • Basal Cell Adhesion Molecule (BCAM) which is a product of alternate splicing of the Lutheran blood group molecule is a part of the immunoglobulin superfamily. (neuromics.com)
  • Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) has been recently identified as a heterophilic ligand for Tim-3. (medscimonit.com)
  • Up coming cells had been treated with a surplus unlabeled LDV formulated with little molecule (1 μm) or substances appealing (10-30 μm) as well as the dissociation from the fluorescent molecule was implemented. (bioinf.org)
  • For kinetic dissociation measurements without inside-out activation cell examples had been preincubated using the fluorescent probe (25 nm ~2 x (12 nm) for the relaxing condition of VLA-4 68 of receptor occupancy (22)) treated with surplus unlabeled LDV formulated with little molecule (1 μm) or substances appealing (10-30 μm) as well as the dissociation from the fluorescent molecule was implemented. (bioinf.org)
  • Dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) and Langerin are C-type lectins of dendritic cells (DCs) that share a specificity for mannose and are involved in pathogen recognition. (uea.ac.uk)
  • Talin and RapL are components of molecular pathways that regulate the avidity of the integrin lymphocyte function-associated antigen 1 (LFA-1) for its ligand, intercellular adhesion molecule 1. (ox.ac.uk)
  • Adhesion molecule pairs of similar height as TCR-pMHC have been argued to facilitate the TCR-pMHC interaction by physically keeping the opposing membranes at an optimal distance for binding. (lu.se)
  • The L1CAM gene provides instructions for producing the L1 cell adhesion molecule protein (shortened to L1 protein), which is found on the surface of nerve cells (neurons) throughout the nervous system. (medlineplus.gov)
  • Kenwrick S, Watkins A, De Angelis E. Neural cell recognition molecule L1: relating biological complexity to human disease mutations. (medlineplus.gov)
  • Inflammatory markers neopterin and soluble intercellular adhesion molecule (sICAM-1) appeared to be clinically useful for isolated assessments of disease activity. (who.int)
  • Leukocyte adhesion deficiency results from an adhesion molecule defect that causes granulocyte and lymphocyte dysfunction and recurrent soft-tissue infections. (msdmanuals.com)
  • Porcine aortic endothelial cell, double-labeled for actin (green) and phospho-tyrosine (red). (weizmann.ac.il)
  • The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. (nature.com)
  • In view of the accelerated vascular disease observed in patients with diabetes, and the enhanced expression of VCAM-1 in diabetic rabbits, we examined whether irreversible advanced glycation endproducts (AGEs), could mediate VCAM-1 expression by interacting with their endothelial cell receptor (receptor for AGE, RAGE). (jci.org)
  • Conclusion: The SLA Ti surface can promote the proliferation and adhesion of endothelial cell and could promote the early healing process of osseointegration. (atlantis-press.com)
  • This was investigated using an in vitro angiogenesis model assessing endothelial cell adhesion to GAG-depleted cartilage explants. (cardiff.ac.uk)
  • This process can occur either through direct contact between cell surfaces such as cell junctions or indirect interaction, where cells attach to surrounding extracellular matrix, a gel-like structure containing molecules released by cells into spaces between them. (wikipedia.org)
  • Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
  • Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
  • Focal adhesion kinase (FAK) is a key integrator of integrin-mediated signals from the extracellular matrix to the cytoskeleton and downstream signaling molecules. (techscience.com)
  • Integrins are transmembrane receptors that mediate cell adhesion by forming links between the extracellular matrix and cytoskeleton. (biologists.com)
  • This formation helps deposition and adhesion of the extracellular matrix on the bone-implant interface. (bvsalud.org)
  • This family of CAMs are membrane proteins that mediate cell-cell adhesion through its extracellular domains and require extracellular Ca2+ ions to function correctly. (wikipedia.org)
  • alpha6 integrin does not appear to mediate adhesion to either substrate. (nih.gov)
  • Prominent among the molecular adhesion components are integrins (β1 and β2), that mediate the interactions of a variety of immune cells to extracellular matrices, and to other immune cells, respectively. (weizmann.ac.il)
  • These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)
  • At the age of defect onset, GPR56 expression is restricted specifically to developing granule cells in the rostral cerebellum, suggesting that GPR56 regulates properties of these cells. (jneurosci.org)
  • cancer cell growth and spreading, it has also been proposed that GPR56 regulates cell adhesion, but this has remained untested in the nervous system. (jneurosci.org)
  • These findings demonstrate that a sub-population of Alix localizes extracellularly and regulates integrin-mediated cell adhesions and fibronectin matrix assembly. (nih.gov)
  • Extracellular microenvironment is highly dynamic where spatiotemporal regulation of cell-instructive cues such as matrix topography tightly regulates cellular behavior. (tue.nl)
  • Tyrosine phosphorylation regulates the adhesions of ras-transformed breast epithelia. (rupress.org)
  • 2010), we discovered that RhoU regulates the cell junctions between cardiomyocytes through the Arhgef7b/Pak kinase pathway in order to guide atrioventricular canal development and cardiac looping. (psu.edu)
  • The CCN family of proteins regulates diverse cellular functions, including cell adhesion , differentiation. (hindlish.com)
  • Lysyl oxidase regulates breast cancer cell migration and adhesion through a hydrogen peroxide-mediated mechanism. (bvsalud.org)
  • Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
  • Attachment of tumor cells to endothelial cells is critical for migration of tumor cells out of the vascular system to establish metastases. (actapress.com)
  • CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
  • We present a simple and quantitative assay system that accurately models human endothelium by use of primary human umbilical vein endothelial cells (HUVECs) in cell culture plates coated with gelatin , a matrix that mimics basal lamina , the matrix that is tightly associated with the vascular endothelium and is critical for its proper function. (bvsalud.org)
  • a4) Activated T-cells form intercellular contacts with each other and cluster through ICAM-1-LFA-1 binding. (weizmann.ac.il)
  • Leukocyte accumulation in response to tryptase may be mediated in part through the selective secretion of IL-8 from endothelial cells. (eurekamag.com)
  • Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. (medscape.com)
  • Thus the infections in patients with leukocyte adhesion deficiency act similarly as those observed in patients with neutropenia. (medscape.com)
  • Labial ulceration from which Escherichia coli was cultured in an 8-month-old girl with leukocyte adhesion deficiency type 1 (LAD I). Note the thin bluish scar at the superior aspect of the labia from an earlier cellulitis. (medscape.com)
  • This 3-year-old girl had leukocyte adhesion deficiency type I (LAD I) with complete absence of CD18 expression. (medscape.com)
  • This 10-month-old patient with severe leukocyte adhesion deficiency type I (LAD I) developed a cervical adenitis caused by Klebsiella pneumoniae. (medscape.com)
  • Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
  • In milder forms of leukocyte adhesion deficiency I (1-30% expression of CD8), patients may survive to adulthood. (medscape.com)
  • Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
  • However, IgM and IgG serum levels are within the reference range in patients with leukocyte adhesion deficiency II. (medscape.com)
  • Leukocyte adhesion deficiency II may be classified as one of the congenital disorders of glycosylation (CDG), a rapidly expanding group of metabolic syndromes with a wide symptomatology and severity. (medscape.com)
  • Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)
  • Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
  • Another reported type of leukocyte adhesion deficiency involves dysfunction in platelet aggregation in addition to a defect in leukocyte adhesion. (medscape.com)
  • Thus, patients with this type of leukocyte adhesion deficiency manifest both severe bacterial infections and bleeding disorder. (medscape.com)
  • This leukocyte adhesion deficiency variant is associated with defective expression of the Rap-1 activator CalDAG-GEFI. (medscape.com)
  • Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1 . (medscape.com)
  • Manifestations of leukocyte adhesion deficiency usually begin in infancy. (msdmanuals.com)
  • Diagnosis of leukocyte adhesion deficiency is by detecting absence or severe deficiency of adhesive glycoproteins on the surface of WBCs using monoclonal antibodies (eg, anti-CD11, anti-CD18) and flow cytometry. (msdmanuals.com)
  • Cells adhesion occurs from the interactions between cell-adhesion molecules (CAMs), transmembrane proteins located on the cell surface. (wikipedia.org)
  • As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
  • Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
  • Nectin proteins have been identified as providing a wide range of functions within the cell. (prospecbio.com)
  • Finally, nectin proteins perform cell-to-cell adhesion. (prospecbio.com)
  • Y. enterocolitica, a common food-borne pathogen with invasive properties, uses the surface proteins invasin and YadA for host cell binding and entry. (uni-muenchen.de)
  • Afterwards, recombinant filamentous bacteriophages which display a short binding motif with a cell adhesive peptide (RGD) on p3 and p8 proteins were immobilized on these 1D Al 2 O 3 nanostructures by a simple dip-coating process to study the cellular response of human endothelial EA hy.926. (bsz-bw.de)
  • Urinalysis demonstrated red bloodstream cells 100/high power field and urine proteins was 3+. (nanoker-society.org)
  • The ras-transformed cells reveal elevated phosphotyrosine in many proteins, including beta-catenin and p120 Cas. (rupress.org)
  • The unc-52 (perlecan), pat-2 α-integrin), and pat-3 (β-integrin) genes encode ECM or transmembrane proteins found at the cell - matrix adhesion sites of both dense bodies and M-lines. (illinois.edu)
  • These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. (hindlish.com)
  • In many T cell activation studies, the SLB contains the nickel-chelating lipid DGS-NTA(Ni) to functionalize the SLB with histidine-tagged proteins. (lu.se)
  • This positioning allows the L1 protein to attach (bind) to other proteins, including other L1 proteins, on neighboring neurons to help these cells stick to one another (cell-cell adhesion). (medlineplus.gov)
  • Other mutations change single protein building blocks (amino acids) in the L1 protein, impairing the protein's ability to interact with other proteins at the cell surface or preventing the protein from reaching the cell surface where it is needed. (medlineplus.gov)
  • Cadherins and IgSF are homophilic CAMs, as they directly bind to the same type of CAMs on another cell, while integrins and selectins are heterophilic CAMs that bind to different types of CAMs. (wikipedia.org)
  • Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. (hindlish.com)
  • Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • To demonstrate differences in human lens epithelial cell adhesion to different intraocular lens biomaterials in vitro and to determine whether these differences can be influenced by coating the intraocular lens surface with commercially available fibronectin. (arvojournals.org)
  • There was a significant difference in the degree of lens epithelial cell attachment to the various types of intraocular lenses in vitro. (arvojournals.org)
  • Some reports suggest that the design of the IOL is important, 10 12 13 others state that the adhesive properties of the lens material may be relevant, 6 7 10 as soft acrylic IOLs show greater lens epithelial cell (LEC) adhesion than do PMMA and silicone lenses. (arvojournals.org)
  • The NECTIN Protein is a family of cell-adhesion molecules that, so far, includes four members. (prospecbio.com)
  • Protein coatings can be prepared in order to take advantage of their biological functions such as specific binding properties and the ability to enhance or reduce cellular adhesion. (doria.fi)
  • BCAM protein is upregulated following malignant transformation of some cell types in vivo and in vitro. (neuromics.com)
  • Context: Adhesion G protein-coupled receptor (GPCR)-G1 (ADGRG1) is the most abundant GPCR in human pancreatic islets, but its role in islet function is unclear. (lu.se)
  • Beneficial effects of collagen type III on β-cell function via activation of the cAMP/protein kinase A pathway, suppression of RhoA and caspase-3 activity, increased β-cell viability, and proliferation were abolished when ADGRG1 was downregulated in β-cells. (lu.se)
  • The proliferation of endothelial cells is detected by MTT method and adhesion of endothelial cells observed by PI fluorescence staining and western-bolt detection of protein of VE-cadherin. (atlantis-press.com)
  • Results: The proliferation of endothelial cells on the titanium surface is better than the culture plate surfaces, the adhesion cells on titanium surface are more numbers, cell morphology is more extended, titanium surface expression of VE-cadherin protein higher than the culture plate surface. (atlantis-press.com)
  • Herewe report the structure of a single-domain bacterial fasciclin I protein, Fdp, in the free-living photosynthetic bacterium Rhodobacter sphaeroides, and show that it confers cell adhesion properties in vivo. (whiterose.ac.uk)
  • This study describes the identification of the unc-112 gene product, a novel, membrane-associated, intracellular protein that colocalizes with integrin at cell-matrix adhesion complexes. (illinois.edu)
  • The protein encoded by this gene may be involved in cell adhesion during neurodegeneration. (hindlish.com)
  • This indicates that TCR-pMHC binding in cell contacts depends significantly on the local environment and not only on the protein-protein interaction per se. (lu.se)
  • The L1 protein spans the cell membrane, with one end of the protein inside the cell and the other end projecting from the outer surface of the cell. (medlineplus.gov)
  • The protein also plays a role in the formation of the protective sheath (myelin) that surrounds certain neurons and the formation of junctions between nerve cells (synapses), where cell-to-cell communication occurs. (medlineplus.gov)
  • The L1CAM gene mutations that cause this condition lead to an L1 protein that cannot facilitate cell-cell adhesion or participate in various neuronal functions. (medlineplus.gov)
  • a1) T cells can also interact with, and be activated by, DCs presenting via MHC/HLA a specific antigen matching the T-cell receptor, and accompanied by co-stimulatory (CD80/CD86 DCs, and CD28 T cells) and adhesive molecules (ICAM-1/LFA-3 DCs, and LFA-1/CD2 T cells). (weizmann.ac.il)
  • T cell immunoglobulin and mucin domain 3 (Tim-3) is a key checkpoint receptor responsible for exhaustion and dysfunction of T cells and plays a critical role in immunosuppression. (medscimonit.com)
  • Furthermore, these results suggest that the sequences comprising peptides GD-3 and GD-4 use an integrin as a receptor, of which the beta 1 integrin subunit is a component for these various cell types. (rupress.org)
  • FLRT family members may function in cell adhesion and / or receptor signalling. (hindlish.com)
  • The interaction between the T cell receptor (TCR) and the antigen-presenting major histocompatibility complex (pMHC) on a contacting T cell and APC, is widely accepted to be the key interaction. (lu.se)
  • In the second part of the thesis, a new method to measure the two-dimensional dissociation constant (2D Kd) of ligand-receptor interactions on single cells is presented. (lu.se)
  • For this purpose, a SLB functionalized with TCR and an adhesion ligand, was allowed to bind cell with pMHC and the corresponding adhesion receptor. (lu.se)
  • Evidence suggests that C1q binds a receptor present on phagocytic cells, termed C1qRP. (medscape.com)
  • Antibody binding to extracellular Alix retards WI38 cell adhesion and spreading on fibronectin and vitronectin. (nih.gov)
  • Alix knockdown in WI38 cells reduces spreading and fibronectin assembly, and the effect is partially complemented by coating recombinant Alix on the cell substratum. (nih.gov)
  • Coating recombinant Alix on the culture substratum for NIH/3T3 cells promotes alpha5beta1-integrin-mediated cell adhesions and fibronectin assembly, and these effects require the aa 605-709 region of Alix. (nih.gov)
  • Coating the lenses with fibronectin caused a significant increase in attachment of lens epithelial cells for all three lens types. (arvojournals.org)
  • Cell attachment was more prominent in the acrylic lenses, but the fibronectin coating negated differences in lens type and caused a significant increase in cell attachment across all groups. (arvojournals.org)
  • Previous studies have shown that small beads coated with FN7-10, a four-domain cell adhesion fragment of fibronectin, bind to cell surfaces and translocate rearward. (biologists.com)
  • Objective: Investigate how ADGRG1 expression and activation by its ligand, collagen III, impacts β-cell function in normal and type 2 diabetic (T2D) islets. (lu.se)
  • Here, we show that the AhR is recruited to target gene enhancers in both ligand (YH439) treated and suspension cells, suggesting a common mechanism of target gene induction between these two routes of AhR activation. (aspetjournals.org)
  • Right here we survey the id of many structurally related substances that were in a position to prevent publicity of ligand-induced binding site (LIBS) epitope following the addition of VLA-4-particular ligand lower binding affinity of VLA-4-particular ligand and stop VLA-4/VCAM-1-reliant cell adhesion. (bioinf.org)
  • This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
  • Other cellular processes regulated by cell adhesion include cell migration and tissue development in multicellular organisms. (wikipedia.org)
  • a2,3) Both DCs and T cells interact with FRCs and their secreted chemokines (CCL19, CCL21), as well as cytokines (IL-6, IL-7), which promote their migration and survival. (weizmann.ac.il)
  • Loss of GPR56 does not affect cell proliferation, migration, or neurite outgrowth. (jneurosci.org)
  • We have studied human melanoma cell (C8161) adhesion and migration in response to stimulation by soluble collagen IV (CIV) using a modified Boyden chamber. (actapress.com)
  • In this modified chamber, shear flow can be introduced over the cell substrate interface affecting tumor cell chemotactic migration through a microporous filter. (actapress.com)
  • This session will focus on experimental and theoretical aspects of prokaryotic and eukaryotic cell adhesion/migration. (confex.com)
  • Papers may also include studies of matrix remodeling (proteolysis, contraction, synthesis), matrix-driven phenotype, or tissue architecture regulation of cellular processes, as well as evaluation of critical biomaterial design parameters for controlling cell adhesion and migration. (confex.com)
  • We discuss how this swimming mode of motility overcomes the physical constraints of microscopic objects moving in fluids, how fat body cells switch on other "motility machinery" to plug the wound on arrival, and whether other cell lineages in Drosophila and other organisms may, under certain circumstances, also adopt swimming as an effective mode of migration. (bris.ac.uk)
  • Here, light responsive liquid crystal polymer networks are used for their adaptive and programmable nature to form hybrid surfaces presenting micrometer scale topographical cues and changes in nanoscale roughness at the same time to direct cell migration. (tue.nl)
  • This study shows that the cell speed and migration patterns are strongly dependent on the height of the (light-responsive) micrometer scale topographies and differences in surface nanoroughness. (tue.nl)
  • Furthermore, switching cell migration patterns upon in situ temporal changes in surface nanoroughness, points out the ability to dynamically control cell behavior on these surfaces. (tue.nl)
  • It is likely that the L1CAM gene mutations that cause these features contribute to disruption of the normal migration and function of nerve cells in the brain and intestine, leading to hydrocephalus and Hirschsprung disease. (medlineplus.gov)
  • In the synthetic niche, interactions with the functionalized scaffold are expected to provide the signals needed for T-cell activation (with or without antigen specificity), lineage selection, proliferation, and survival. (weizmann.ac.il)
  • Our results suggest that transmigration of C8161 cells under flow conditions can be influenced by PMNs, mediated by Mac 1/ICAM-1 adhesive interactions and enhanced by altered cytokine production. (actapress.com)
  • Appropriate papers include those addressing fundamental molecular mechanisms of cell-cell and cell-matrix/surface interactions in two or three dimensional structures, as well as the roles of these cellular processes in tissue engineering, cell physiology or biotechnology. (confex.com)
  • Cell Adhesion: the process by which cells interact and attach to a surface, substrate or another cell, mediated by interactions between molecules of the cell surface. (labroots.com)
  • Controlling the cell-substrate interactions is important in e.g. the development of medical devices and platforms for the cytotoxicity assays and biofilm studies. (doria.fi)
  • These cells exhibit altered cell-cell and cell-substrate interactions. (rupress.org)
  • Here we have identified changes in the adhesions and cytoskeletal interactions of transformed epithelial cells that contribute to their altered morphology. (rupress.org)
  • Contributing to the modified adherens junctions of the transformed cells are decreased interactions among beta-catenin, E-cadherin, and the actin cytoskeleton. (rupress.org)
  • Interactions between immune cells such as T cells and antigen-presenting cells (APCs) are integral for mounting an adaptive immune response. (lu.se)
  • In the first part of this thesis I show that interactions between DGS-NTA(Ni) and the T cells can lead to, unwanted, T cell signaling. (lu.se)
  • The DGS-NTA(Ni) signaling was argued to be due to TCR-DGS-NTA(Ni) interactions and stressed the importance of adequately blocking these interactions in T cell activation studies. (lu.se)
  • interactions between cells. (lu.se)
  • these glycoproteins facilitate cellular interactions, cell attachment to blood vessel walls, cell movement, and interaction with complement fragments. (msdmanuals.com)
  • abstract = "Several cell lineages migrate through the developing and adult tissues of our bodies utilising a variety of modes of motility to suit the different substrates and environments they encounter en route to their destinations. (bris.ac.uk)
  • The CD11/CD18 complex is part of the beta-2 integrin family and is important in adhesion and phagocytosis (see Table 1). (medscape.com)
  • I. Blood methanol concentration and neural cell adhesion molecules. (cdc.gov)
  • In this study, we examined the effects of FAK point mutations at tyrosine residues (Y397, Y925, Y861, and Y576/7) on osteogenic differentiation of human mesenchymal stem cells exposed to collagen I and cyclic tensile strain. (techscience.com)
  • 2 (2007) observed that cell adhesion and proliferation, as well as the osteogenic differentiation of mouse mesenchymal stem cells (MSCs) to Ti discs were significantly similar to those on the plastic surface of the culture, indicating Ti as an excellent material for repairing hard tissue in the field of bone tissue engineering. (bvsalud.org)
  • The term "adhesion" to the biomaterial refers to the most important phase, since the quality of it will influence morphology and the capacity of cell proliferation and differentiation 3 . (bvsalud.org)
  • Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
  • The recombinant phage overcoating also supports an isotropic cell spreading rather than elongated cell morphology as we observed on as-deposited Al 2 O 3 1D nanostructures. (bsz-bw.de)
  • Transformed epithelial cells often are characterized by a fibroblastic or mesenchymal morphology. (rupress.org)
  • Inhibitors of tyrosine kinases decrease the level of tyrosine phosphorylation and restore a normal epithelial morphology to the ras-transformed cells. (rupress.org)
  • Molecular composition map" of focal adhesions and stress fibers. (weizmann.ac.il)
  • Using MCF-10A human breast epithelial cells as a model system, we have found that transformation by an activated form of ras is characterized by less developed adherens-type junctions between cells but increased focal adhesions. (rupress.org)
  • The initial tissue reaction to infection is a focal, intensely suppurative necrosis consisting largely of accumulations of polymorphonuclear leukocytes, followed by invasion of macrophages, epithelioid cells, and lymphocytes. (cdc.gov)
  • Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. (wikipedia.org)
  • The family of molecules will primarily regulate adhesion between two cells, although Nectin-like molecules - known as Necl - will perform other tasks at a cellular level. (prospecbio.com)
  • Failure to properly form these cell adhesions during cardiac development may lead to structural heart defects and mechanistically account for the cellular events that occur in certain human congenital heart diseases. (psu.edu)
  • Antibodies against the beta 1 integrin subunit inhibited HT-1080 cell adhesion and neurite outgrowth on surfaces adsorbed with peptides GD-3 and GD-4. (rupress.org)
  • Aim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on different titanium surfaces. (bvsalud.org)
  • Therefore, the present study aimed to evaluate the adhesion capacity of mouse bone marrow MSCs to smooth and plasmanitrided Ti surfaces in the cathodic cage configuration. (bvsalud.org)
  • Cell adhesion is the process by which cells interact and attach to neighbouring cells through specialised molecules of the cell surface. (wikipedia.org)
  • These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
  • We also have determined that UNC-112 is required to organize PAT-3/β-integrin after it is integrated into the basal cell membrane, but is not required to organize UNC-52/perlecan in the basement membrane, nor for DEB-1/vinculin to localize with PAT-3/β-integrin. (illinois.edu)
  • Furthermore, UNC-112 requires the presence of UNC-52/perlecan and PAT-3/β- integrin, but not DEB-1/vinculin to become localized to the muscle cell membrane. (illinois.edu)
  • a) Lymph node-residing and infiltrating T-cells and dendritic cells (DC) migrate on a 3D network of fibroblastic reticular cells (FRCs) and their associated ECM. (weizmann.ac.il)
  • In the natural infection, DV is introduced into human skin by an infected mosquito vector where it is believed to target immature dendritic cells (DCs) and Langerhans cells (LCs). (unboundmedicine.com)
  • CR2 binds C3dg, and C3d is present on B cells and dendritic cells (see the Table 1). (medscape.com)
  • In short-term adhesion assays, the preferred substrate for HCE-T attachment is placental laminin. (nih.gov)
  • On the ventral surface this rearward movement of the cytoskeleton contributes to forward motion of the cell relative to the substrate. (biologists.com)
  • Investigations Bloodstream investigations uncovered high white cell count number of 24.39109/L, regular haemoglobin (12.5?g/dL) level and regular platelet (424×109/L) count number. (nanoker-society.org)
  • Instead of classical cadherins, non-classical cadherins such as desmogleins and desmocollins act as adhesion molecules and they are linked to intermediate filaments instead of actin filaments. (wikipedia.org)
  • p120 Cas, whether tyrosine phosphorylated or not, partitions into the detergent soluble fraction, suggesting that it is not tightly bound to the actin cytoskeleton in either the normal or ras-transformed cells. (rupress.org)
  • Fluorescence microscopy showed that the trimer and pentamer bound strongly to the cell surface, and within 5 minutes were prominently localized to actin fiber bundles. (biologists.com)
  • These links are used in cell motility as the actin cytoskeleton generates force pulling the integrin and attached matrix molecules towards the cell center. (biologists.com)
  • Myosin and actin are not organized into sarcomeres in the body wall muscle cells of these mutants, and dense body and M-line components fail to assemble. (illinois.edu)
  • Synthetic peptides from the carboxy-terminal globular domain of the A chain of laminin: their ability to promote cell adhesion and neurite outgrowth, and interact with heparin and the beta 1 integrin subunit. (rupress.org)
  • residues 2,110-3,060) of the A chain of murine-derived laminin has been shown to promote heparin binding, cell adhesion, and neurite outgrowth. (rupress.org)
  • Epithelial cells growing on a patterned adhesive surface with the shape of the Weizmann Institute tree. (weizmann.ac.il)
  • In our current attempts to create a synthetic "immune niche," we employ diverse surface patterning approaches that might mimic "partnering cells", which are regularly located in the immune niches, and replace them in the process of T-cell activation (Figure 1). (weizmann.ac.il)
  • The cell growth of ARPE-19, HepG2 and Staphylococcus aureus on paper substrates with different surface characteristics was studied and printability of viable cells was tested. (doria.fi)
  • We propose that DC-SIGN concentrates mosquito-derived DV particles at the cell surface to allow efficient interaction with an as yet unidentified entry factor that is ultimately responsible for DV internalization and pH-dependent fusion into DCs. (unboundmedicine.com)
  • To observe the proliferation and adhesion of umbilical cord endothelial cells (HUVEC) on the titanium (Ti) surface with large particles blasted-etched (SLA) treatments and further clarify the mechanism of action of each other between the Ti implant surface and the body. (atlantis-press.com)
  • Methods: The human umbilical cord endothelial cells are cultured on the surface of SLA-Ti disks and culture plates. (atlantis-press.com)
  • A novel synthesis of a nanostructured cell adhesive surface is investigated for future stent developments. (bsz-bw.de)
  • Surface modification can also affect properties that promote cell adhesion . (hindlish.com)
  • Conclusions: It was demonstrated that titanium surface treatment with ionic nitriding in a cathodic cage is biocompatible since it preserved the integrity of the cultivated MBMMC for a period of 24 h, allowing their adhesion. (bvsalud.org)
  • The foregoing properties allow the scarring and maintenance of tissue structure adhesion to the Ti surface 1 . (bvsalud.org)
  • These properties determine the adhesion and behavior of cells in contact with the surface. (bvsalud.org)
  • Interference RNA-mediated knockdown of GPR56 recapitulates the loss of adhesion seen in knock-outs, and reexpression of GPR56 rescues the adhesion defect in knock-out granule cells. (jneurosci.org)
  • Furthermore, we aim at creating conditions that will enable us to develop an effective ex vivo stimulation system, tailored for different subclasses of T cells, including helper CD4 + cells, cytotoxic (CD8 + ) T cells, and T regulatory (T-Reg) cells. (weizmann.ac.il)
  • After optimization , we were able to detect increased adhesion of the THP-1 cells to the endothelium in response to the inflammatory cytokine TNFα in a dose -dependent manner like what has been observed in vivo. (bvsalud.org)
  • citation needed] Each of these adhesion molecules has a different function and recognizes different ligands. (wikipedia.org)
  • b) In a novel approach for construction of a synthetic immune niche, many of the cell-bound and ECM-associated ligands are transferred to a synthetic 2D (left) or 3D scaffold (right), to which T cells can bind. (weizmann.ac.il)
  • When co-cultured with human mesenchymal stem cells (hMSCs), the NMs exhibit excellent bioactivity in encouraging hMSCs adhesion. (jove.com)
  • When co-cultured with human mesenchymal stem cells, the nano-matrix exhibited excellent bioactivity in encouraging cell adhesion. (jove.com)
  • mesenchymal stem cells, bone marrow, titanium. (bvsalud.org)
  • If the interaction is favourable, then T cell activation occurs. (lu.se)
  • While the cell density decreased on as-deposited 1D Al 2 O 3 nanostructures, we observed enhanced cell proliferation and cell-cell interaction on recombinant phage overcoated 1D Al 2 O 3 nanostructures. (bsz-bw.de)
  • VCAM1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 682 amino acids (25-698 a.a) and having a molecular mass of 75.4kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). (angioproteomie.com)
  • Briefly, IgSFs are adhesion molecules that bind to themselves (homophilic) or compatible IgSFs (heterophilic) across cell-cell junctions. (elifesciences.org)
  • C3b and C4b bind to CR1, which is present on various phagocytes and also on erythrocytes and B cells. (medscape.com)
  • Pre-adsorbed avidin inhibited bacterial adhesion on two-component latex. (doria.fi)
  • This plasmid encodes both bundle-forming pili (Bfp) that promote bacterial adherence to mammalian cells and are required for virulence ( 4 ) and a transcriptional activator, known as Per, that upregulates genes, such as eae , within a pathogenicity island termed the locus for enterocyte effacement (LEE) ( 5 ). (cdc.gov)
  • We have investigated the proinflammatory actions of the major mast cell product tryptase on HUVEC. (eurekamag.com)
  • Addition of purified tryptase was found to stimulate thymidine incorporation, but induced little alteration in cell numbers, suggesting it is not a growth factor for HUVEC. (eurekamag.com)
  • We describe using this system to quantitatively measure adhesion of the inflammatory cells - monocytic THP-1 cell line to the HUVEC monolayer. (bvsalud.org)
  • We established an intracranial GBM model using C57BL/6 mice and GL261 cells, and treated the mice with single or combined monoclonal antibodies (mAbs) against Tim-3/CEACAM1. (medscimonit.com)
  • In addition, solutions of these peptides or antibodies generated against these peptides inhibited laminin-mediated HT-1080 cell adhesion. (rupress.org)
  • Therefore, many laboratories try with great effort to develop resorbable tissue scaffolds that could support the patient´s cells. (nature.com)
  • ECM provides mechanical support for cells and also determines the shape of tissue 9 . (nature.com)
  • Many enteric pathogens are equipped with multiple cell adhesion factors which are important for host tissue colonization and virulence. (uni-muenchen.de)
  • of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
  • These studies establish a novel role for GPR56 in the adhesion of developing neurons to basal lamina molecules and suggest that this adhesion is critical for maintenance of the pia and proper cerebellar morphogenesis. (jneurosci.org)
  • Defects in cell adhesion are usually attributable to defects in expression of CAMs. (wikipedia.org)
  • Our experiments demonstrate that GPR56 is essential for proper morphogenesis of the rostral cerebellum and suggest that defects caused by loss of GPR56 arise from granule cell abnormalities in this region. (jneurosci.org)
  • These results support a novel hypothesis that the cerebellar defects in Gpr56 knock-outs, and potentially in human patients with GPR56 mutations, arise from the disrupted adhesion of developing granule cells to the pial BM. (jneurosci.org)
  • Phagocytic cell defects Immunodeficiency disorders are associated with or predispose patients to various complications, including infections, autoimmune disorders, and lymphomas and other cancers. (msdmanuals.com)
  • Exposure of cultured human ECs to AGEs induced expression of VCAM-1, increased adhesivity of the monolayer for Molt-4 cells, and was associated with increased levels of VCAM-1 transcripts. (jci.org)
  • Design: Genes associated with the ADGRG1 in human islets was probed by RNA-sequencing of human pancreatic islet isolated from cadaveric donors, followed by functional studies on β-cell proliferation, apoptosis, and insulin secretion in human and mouse islets and in INS-1 cells. (lu.se)
  • Results: ADGRG1 is the most abundant GPCR mRNA in both human and mouse islets, and its expression inhumanislets strongly correlates with genes important for β-cell function and T2 Drisk. (lu.se)
  • Unique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis. (uni-muenchen.de)
  • In this study, we demonstrate unique cell adhesion and invasion properties of Y. enterocolitica serotype O:3 strains, the most frequent cause of human yersiniosis, and show that these differences are mainly attributable to variations affecting the function and expression of invasin in response to temperature. (uni-muenchen.de)
  • Like their human counterpart, these large cells were previously presumed to be immotile. (bris.ac.uk)
  • Bovine cartilage explants were treated with hyaluronidase to deplete GAG content and then seeded with fluorescently tagged human endothelial cells (HMEC-1). (cardiff.ac.uk)
  • The focus of the present study was to examine the adhesion of human LECs to different IOL biomaterials. (arvojournals.org)
  • 1987. The effect of electrically generated silver ions on human cells. (cdc.gov)
  • Type 3 is caused by mutations in the FERMT3 gene (11q13.1), which encodes kindlin-3 in hematopoietic cells. (msdmanuals.com)
  • Cadherins forms homophilic attachment between themselves, which results in cells of a similar type sticking together and can lead to selective cell adhesion, allowing vertebrate cells to assemble into organised tissues. (wikipedia.org)
  • The THP-1 cells are fluorescently labeled which allows to quantify the number of the fluorescent THP-1 cells adhering to the endothelium under a microscope and the level of florescence - a quantitative measure of the number of adhering fluorescent THP-1 cells using a fluorescent plate reader. (bvsalud.org)