• Growth on CO requires the enzyme carbon monoxide dehydrogenase (CODH) that catalyzes the reversible conversion between CO and CO 2 . (hindawi.com)
  • The systematic name of this enzyme class is formate:NADP+ oxidoreductase. (wikipedia.org)
  • menaquinone oxidoreductase reaction, as the major mode of energy conservation, rather than formate or H 2 cycling during respiratory growth. (hindawi.com)
  • The purified ReBDH in the native form was found to exist as a monomer with a calculated subunit size of 37180, belonging to the family of the zinc-containing alcohol dehydrogenases. (mdpi.com)
  • alcohol dehydrogenase 5 (class III), chi. (wikigenes.org)
  • In humans and monkeys, the conversion to formaldehyde is mediated by alcohol dehydrogenases and basically limited to the capacity of those enzymes. (europa.eu)
  • U. Ryde, L. Hemmingsen (1997) "The active-site metal coordination geometry of cadmium-substituted alcohol dehydrogenase. (lu.se)
  • Artificial microRNA technology was investigated as a means of down regulating metabolic pathways in the green alga Chlamydomonas reinhardtii, targeting pyruvate formate lyase (PFL1), which catalyzes the conversion of pyruvate to acetyl-CoA and formate during anoxic conditions. (ed.ac.uk)
  • Formate is an ideal electron donor for cofactor regeneration due to its general availability, low reduction potential, and benign byproduct (CO 2 ). (biorxiv.org)
  • However, formate dehydrogenases (FDHs) are usual specific to NAD + , such that NADPH regeneration with formate is challenging. (biorxiv.org)
  • By selecting for growth with formate as sole NADPH source, we isolate several enzyme variants that support efficient NADPH regeneration. (biorxiv.org)
  • Engineering a Formate Dehydrogenase for NADPH Regeneration. (nih.gov)
  • This study aimed to prepare robust immobilized formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. (arel.edu.tr)
  • An air-stable formate dehydrogenase (FDH), an enzyme that catalyzes the oxidation of formate to carbon dioxide, was purified from the sulfate reducing organism Desulfovibrio gigas (D. gigas) NCIB 9332. (unl.pt)
  • Formate de-hydrogenases (FDHs) catalyze the two-electron oxidation of formate to carbon dioxide. (iucr.org)
  • In this work, the co-immobilization of formate dehydrogenase (FDH) and glycerol dehydrogenase (GlyDH) enzymes is proposed to reduce CO 2 into formic acid, an important chemical intermediate. (pucv.cl)
  • As expected, domain 1 shares the same Rossmann fold as the related enzymes, methionyl-tRNA-formyltransferase and glycinamide ribonucleotide formyltransferase, but, unexpectedly, the structural similarity between the amino-terminal domain of 10-formyltetrahydrofolate dehydrogenase and methionyl-tRNA-formyltransferase extends to the C terminus of both proteins. (rcsb.org)
  • In the absence of electron acceptor, downregulation of F 420 H 2 dependent steps of the acetyl-CoA pathway is linked to transient formate generation. (hindawi.com)
  • In enzymology, a formate dehydrogenase (NADP+) (EC 1.17.1.10) is an enzyme that catalyzes the chemical reaction formate + NADP+ ⇌ {\displaystyle \rightleftharpoons } CO2 + NADPH Thus, the two substrates of this enzyme are formate and NADP+, whereas its two products are CO2 and NADPH. (wikipedia.org)
  • To further probe the system, we generated a double mutant (pfl1-1 adh1) that is unable to synthesize both formate and ethanol. (montana.edu)
  • Whereas wild-type Chlamydomonas fermentation primarily produces formate and ethanol, the double mutant reroutes glycolytic carbon to lactate and glycerol. (montana.edu)
  • Nuclear magnetic resonance spectroscopy confirmed a substantial decrease in the production of formate in the knockdown lines during dark anoxic conditions and a re-routing of metabolism leading to enhanced production of ethanol and lactate. (ed.ac.uk)
  • Under microaerobic conditions in the light, induced by sulphur-deprivation, knock-down of PFLI resulted in reduced formate and ethanol production, increased net consumption of acetate and the excretion of lactate but no increase in the production of hydrogen. (ed.ac.uk)
  • The immobilization yield of formate dehydrogenase and glycerol dehydrogenase is around 100% with all the supports studied. (pucv.cl)
  • The gene encoding a (2 R ,3 R )-2,3-butanediol dehydrogenase from Rhodococcus erythropolis WZ010 (ReBDH) was over-expressed in Escherichia coli and the resulting recombinant ReBDH was successfully purified by Ni-affinity chromatography. (mdpi.com)
  • The protein comprises two functional domains: a hydrolase domain that removes a formyl group from 10-formyltetrahydrofolate and a NADP(+)-dependent dehydrogenase domain that reduces the formyl to carbon dioxide. (rcsb.org)
  • Here, we harness the power of natural selection to engineer FDH variants to simultaneously optimize three properties: kinetic efficiency with NADP + , specificity towards NADP + , and affinity towards formate. (biorxiv.org)
  • NAD and NADP are coenzymes for dehydrogenase-type reactions. (medscape.com)
  • Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate. (mpg.de)
  • This vitamin is a cofactor for pyruvate dehydrogenase in the Krebs cycle. (medscape.com)
  • 10-Formyltetrahydrofolate dehydrogenase (FDH) converts 10-formyltetrahydrofolate, a precursor for nucleotide biosynthesis, to tetrahydrofolate. (rcsb.org)
  • We find that the kinetically superior enzyme variant, harboring five mutations, has 5-fold higher efficiency and 13-fold higher specificity than the best enzyme previously engineered, while retaining high affinity towards formate. (biorxiv.org)
  • Schauer, N.L. and Ferry, J.G. FAD requirement for the reduction of coenzyme F 420 by formate dehydrogenase from Methanobacterium formicicum . (enzyme-database.org)
  • Growth of E. coli on formate and methanol via the reductive glycine pathway. (mpg.de)
  • We found that the tricarboxylic acid cycle-associated enzyme α-ketoglutarate (α-KG) dehydrogenase (KGDH) entered the nucleus, where it interacted with various JMJs to regulate α-KG-dependent histone demethylations by JMJs, and thus controlled genome-wide gene expression in plants. (bvsalud.org)
  • On the other hand, transient formate formation was observed in cultures grown on CO and sulfate [ 2 ], but the enzymatic rationale supporting formate cycling as a mechanism for energy conservation is still lacking. (hindawi.com)
  • Light alters histone methylation in plants via nuclear α-ketoglutarate dehydrogenase. (bvsalud.org)
  • Expression of Formate-Tetrahydrofolate Ligase Did Not Improve Growth but Interferes With Nitrogen and Carbon Metabolism of Synechocystissp. (mpg.de)
  • The mammalian metabolism of methanol occurs mainly in the liver, where methanol is initially converted to formaldehyde, which is in turn converted to formate. (europa.eu)
  • Furthermore, a great increase in the retained activity for the formate dehydrogenase enzyme was noted, passing from 18% to 89%, when the mesostructured clinoptilolite was used as support. (pucv.cl)
  • Es in formate dehydrogenase activity. (heme-oxygenase.com)
  • The structure also provides clues as to how, in the native enzyme, the hydrolase domain transfers its product to the dehydrogenase domain. (rcsb.org)
  • The enzyme, characterized from methanogenic archaea, is involved in formate-dependent H 2 production. (enzyme-database.org)
  • We also develop new tools to activate CO2 in vivo, in a collaborative project with Malin Bomberg (VTT) and Jan Deska (University of Helsinki), where we study acid-tolerant formate dehydrogenases. (aalto.fi)
  • In their study of 38 patients from a Czech methanol mass poisoning in 2012, S-formate levels ≥3.7 mmol/L were seen to lead to the first clinical signs of visual toxicity, indicating hemodialysis. (medscape.com)
  • Beside utilizing CO, A. fulgidus grows with H 2 and formate and a wide variety of simple and complex organic compounds [ 4 - 7 ]. (hindawi.com)
  • After repeated inhalation of 2.6 mg/L for 6 hours/day, 5 days/week, for 1 or 2 weeks, monkeys showed no discernible increase in formate concentration in blood (estimated body burden 200 to 300 mg/kg bw/d). (europa.eu)