Ionomycin is a highly specific ionophore isolated from Streptomyces conglobatus . (agscientific.com)
Stimulation18
PMA ionomycin stimulation is a technique used to activate T cells. (reportergene.com)
PMA ionomycin stimulation is often used to induce T cell proliferation and cytokine production. (reportergene.com)
PMA ionomycin stimulation is a powerful tool for studying T cell biology and for developing new treatments for T cell-mediated diseases. (reportergene.com)
In research, PMA ionomycin stimulation is used to study the signaling pathways that regulate T cell activation and differentiation. (reportergene.com)
In medicine, PMA ionomycin stimulation is used to activate T cells for adoptive cell therapy. (reportergene.com)
PMA ionomycin stimulation is a relatively safe and effective technique for activating T cells. (reportergene.com)
PMA ionomycin stimulation can induce the production of reactive oxygen species (ROS), which can damage cells. (reportergene.com)
PMA ionomycin stimulation can also induce the production of inflammatory cytokines, so it is important to monitor cells for signs of inflammation. (reportergene.com)
Overall, PMA ionomycin stimulation is a valuable tool for studying and manipulating T cells. (reportergene.com)
Mechanism of action: PMA ionomycin stimulation works by activating a variety of signaling pathways in T cells. (reportergene.com)
Differentiation of T cells: PMA ionomycin stimulation can be used to differentiate T cells into specific subsets. (reportergene.com)
Research: PMA ionomycin stimulation is used to study the role of T cells in a variety of diseases, including autoimmune diseases, infectious diseases, and cancer. (reportergene.com)
For example, researchers have used PMA ionomycin stimulation to study how T cells contribute to the development of inflammatory bowel disease. (reportergene.com)
Medicine: PMA ionomycin stimulation is used to treat a variety of T cell-mediated diseases, including psoriasis, rheumatoid arthritis, and multiple sclerosis. (reportergene.com)
For example, researchers are currently investigating the use of PMA ionomycin stimulation to treat psoriasis. (reportergene.com)
F ) Analysis of IL-17A and IL-13 production by ILCs from 3mo mice as identified in Panel A after PdBU/ionomycin stimulation. (elifesciences.org)
In vitro CD4+ (E) and CD8+ (F) T cells production of TNF, IL-2, IFN, Granzyme B (GZB) and IL-17, after unspecific stimulation with PMA/ionomycin, before and after nivolumab injection in patient 3. (cdc.gov)
The expression of Egr2 and FasL, and the phosphorylation of AKT and ERK, after ionomycin and PMA co-stimulation, was detected, while the Ca 2+ mobilization stimulated by K + solution was determined. (researchsquare.com)
Phorbol2
Additional activators of lymphocyte proliferation are phorbol myristate acetate (PMA) with ionomycin or anti-CD3 and anti-CD28. (medscape.com)
Washed cells were stimulated with either plate-bound anti-CD3ε, or phorbol 12-myristate 13-acetate (PMA) and ionomycin, for 4 hours. (biomedcentral.com)
Calcium2
Ionomycin increases intracellular calcium levels, which also activates a number of signaling pathways. (reportergene.com)
The present work deals with the characterization of the proteins which are secreted upon activation of human neutrophils by ionomycin and calcium. (nih.gov)
Intracellular1
Ionomycin is also used to stimulate the intracellular production of the cytokines, interferon, perforin, IL-2 and IL-4 usually in conjunction with PMA. (agscientific.com)
Activation3
Production of cloned horse foals using roscovitine-treated donor cells and activation with sperm extract and/or ionomycin. (tamu.edu)
Parthenogenetic activation of metaphase II (MII) oocytes along with a mix of activating agents including ionomycin and 6-dimethylaminopurine (6-DMAP), stops the protrusion of the second polar body thereby allowing the activated oocytes to retain their entire genetic material. (pharmaceutical-technology.com)
However, when combined with activating agents ionomycin and puromycin, parthenogenetic activation of MII oocytes allows the second polar body to extrude. (pharmaceutical-technology.com)
Cells6
By using different concentrations of PMA and ionomycin, it is possible to induce T cells to differentiate into the desired subset. (reportergene.com)
For example, it is reported that ionomycin promotes apoptosis in immature B cells and regulates cell cycle in mature B cells. (agscientific.com)
G ) Muzzle-infiltrating cells were isolated from LMC and Sox13 -/- mice and re-stimulated in vitro with PdBu/ionomycin to assess production of IL-5 and IL-13 by ILCs. (elifesciences.org)
Skin cells were stimulated with PdBU/ionomycin and IL-17 production assessed in CD3+ T cells. (elifesciences.org)
D ) Muzzle-infiltrating cells were isolated from indicated mice, stimulated in vitro with PdBu/ionomycin, and analyzed for αβ T cell subset-specific production of IL-17A and IL-22 and for CD4 + T cell production of IL-4, and IL-13. (elifesciences.org)
Chronic culture of murine T cells in TNF does not alter the stability of IL-2 mRNA induced via TCR, or by PMA and ionomycin, in those cells. (biomedcentral.com)
Important2
It is important to follow established protocols and to use the appropriate concentrations of PMA and ionomycin. (reportergene.com)
PMA and ionomycin are both toxic compounds, so it is important to handle them with care. (reportergene.com)
Phorbol myristate acetate2
In contrast, response to anti-CD3, superantigens, and phorbol myristate acetate (PMA)/ionomycin may be detectable. (medscape.com)
IRS cell function was confirmed by treatment with phorbol myristate acetate (PMA) and ionomycin to induce activation and thus the expression of FP2. (bmj.com)
Induce1
In undifferentiated HL-60 cells, neither fMLP nor ionomycin induce significant inositol phosphate formation, and the increase in [Ca++]i elicited by ionomycin is transient. (aspetjournals.org)
Induces2
Receptor-independent stimulation of Tgfb1(-/-) T cells by PMA plus ionomycin induces IL-2 production and mitogenic response, and it rescues them from anergy. (nih.gov)
Ionomycin also induces a rapid but sustained increase of [Ca++]i. (aspetjournals.org)
Nigericin1
Incubation of cells for 70 min in culture medium in the presence of ionomycin plus nigericin led to a very significant 3- or 2-fold increase in potassium in the electron-dense vacuoles and the iron-rich vacuoles, respectively, with no significant change in the other elements investigated. (nih.gov)
Antibiotic that binds1
Ionomycin is an ionophoric antibiotic that binds Pb2+ and Ca2+, and serves as an effective mobile carrier of both cations. (sigmaaldrich.com)
12. Ionomycin downregulates beta-catenin/Tcf signaling in colon cancer cell line. (nih.gov)
Results As expected, live cell fluorescence imaging revealed that PMA/ionomycin treatment induced expression of FP2. (bmj.com)
Addition1
The human AITR expression of is not induced by the treatment with dexamethasone as found in mouse species, but is controlled by antigen-receptor interaction or by soluble anti-CD3 and anti-CD28 or PMA with addition of ionomycin. (prospecbio.com)
Combination1
PHA or PMA in combination with ionomycin. (medscape.com)
Free1
Ionomycin is often sold as a free acid, or as a Ca2+ salt. (wikipedia.org)