Jurkat cells. Medical search. Frequent questions

WB: Jurkat whole cell lysate. (abcam.com)
Lysate from Jurkat cells was probed with anti-CD3ζ (clone 8D3, Comp. (bdbiosciences.com)
Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. (bdbiosciences.com)
Detection:66169-1-Ig 1:500) with HeLa cells lysate 3200ug. (ptglab.com)
Western blotting analysis of human CD81 using mouse monoclonal antibody M38 on lysate of Jurkat cell line under non-reducing conditions. (exbio.cz)

These results indicated that oxidative stress induces PKR expression essentially via the IFN-γ activation signal, and causes apoptosis in Jurkat T cells. (korea.ac.kr)

J.RT3-T3.5 cells have a mutation in the T cell receptor beta chain locus precluding expression of this chain. (wikipedia.org)
they do not express surface CD3 or produce the T cell receptor alpha/beta heterodimer. (wikipedia.org)
Since they are deficient in the TCR complex, these cells are a useful tool for transfection studies using T cell receptor alpha and beta chain genes and are widely used in labs in which T cell receptor gene transfer technologies are studied. (wikipedia.org)
The D1.1 cell line does not express the CD4 molecule, an important co-receptor in the activation pathway of helper T cells. (wikipedia.org)
The J.gamma1 subline contains no detectable phospholipase C-gamma1 (PLC-γ1) protein and therefore has profound defects in T cell receptor (TCR) calcium mobilization and activation of nuclear factor of activated T cells (NFAT, an important transcription factor in T cells). (wikipedia.org)
Jurkat cells are susceptible to HIV infection since they have the CD4 T-cell receptor (TCR) that is required for HIV attachment and infection of this retrovirus. (oxinst.com)
SR-F1 is now viewed as a multifaceted receptor ( 9 ), which can bind and mediate cell internalization of a wide range of endogenous and exogenous ligands, suggesting key roles in immune responses and tissue homeostasis. (frontiersin.org)
All membrane-bound splice variants showed receptor activity toward fluorescent AcLDL when expressed transiently in COS-1 cells ( 10 ). (frontiersin.org)
Background: The leukaemia-derived Jurkat E6.1 cell line has been used as a model T cell in the study of many aspects of T cell biology, most notably activation in response to T cell receptor (TCR) engagement. (ox.ac.uk)
Tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a role in diffuse large B cell lymphoma (DLBCL) biological processes.TRAF6 Stable Knockdown HT Cell Line was generated via lentivirus transduction with TRAF6 shRNA plasmids, and the transfectant was selected with puromycin. (topsan.org)
Multistep mutations in the T-cell receptor (TCR), STAT3, and NOTCH pathways establish clone-specific transcriptomic abnormalities and further accelerate their proliferative potential to develop highly malignant clones, leading to disease onset and progression. (nature.com)
A hallmark of causative driver lesions was their enrichment in the components of the T cell receptor (TCR)/nuclear factor (NF)-κB signaling pathway. (nature.com)
Once the Fc receptor binds to the Fc region of IgG, the Natural Killer cell releases cytokines such as IFN-γ and cytotoxic molecules that attack the pathogen-infected target cell. (cobioer.com)
Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. (nih.gov)
The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multi-component complex responsible for recognizing antigen in the context of MHC molecules. (bdbiosciences.com)
The 8D3 antibody recognizes the ζ chain of the human and mouse T cell antigen receptor-associated CD3 complex. (bdbiosciences.com)
simultaneously bind to major histocompatibility complex published a study of seven patients with severe streptococ- class II molecules and T-cell receptor molecules bearing a cal infections: SPE-A was detected in serum samples from four patients (27). (cdc.gov)

The JCaM1.6 cell line is deficient in Lck activity due to the deletion of part of the LCK gene (exon 7) from the LCK transcript. (wikipedia.org)
In conclusion, for the first time, we demonstrated PBR expression in Jurkat cells: the protein bound classic PBR ligands with micromolar affinity constants and presented a modified amino acidic sequence consequent to the detection of two gene polymorphisms. (unipi.it)
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, low affinity (F158) variant and Fcγ chain. (cobioer.com)
Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI maps remains unresolved. (thebiogrid.org)
Jurkat T cells were transfected with plasmids expressing the CMV IE gene products or with a control plasmid. (jci.org)
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations. (qiagen.com)
This gene is downregulated in many cancer types and may be involved in the inhibition of cell proliferation. (nih.gov)
Benzo[a]pyrene reduced promoter methylation and increased gene expression of the same gene in Jurkat and normal prostate epithelial cells. (cdc.gov)

T6164SIRT7 Stable Knockdown HeLa Cell Line was generated by lentiviral driven expression of shRNAs. (topsan.org)
ICC/IF: HeLa, human adenocarcinoma, human schlemms canal endothelium and wild-type HAP1 cells. (caslab.com)
Flow Cyt (intra): HeLa cells. (caslab.com)

17. NOTCH1 and SOX10 are Essential for Proliferation and Radiation Resistance of Cancer Stem-Like Cells in Adenoid Cystic Carcinoma. (nih.gov)
CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. (exbio.cz)
Overexpression of miR-338-3p in MM cells significantly inhibited proliferation and promoted apoptosis, caspase 3, and caspase 8 activity. (techscience.com)
Of note, the restoration of CDK4 expression markedly abolished the effect of miR-338-3p overexpression on cell proliferation, apoptosis, caspase 3, and caspase 8 activities in MM cells. (techscience.com)
OBJECTIVE: To investigate the effects of methionine restriction on proliferation, cell cycle and apoptosis of human acute leukemia cells. (bvsalud.org)
METHODS: Cell Counting Kit-8 (CCK-8) assay was used to detect the effect of methionine restriction on HL-60 and Jurkat cells proliferation. (bvsalud.org)

T6418TEM1 Knockdown Stable NIH3T3 Cell Line was established via transfection of TEM1 shRNA-expressing vector (pLKO.1-TEM1) into parental NIH3T3 cells. (topsan.org)
Cell-type-specific protocols using HiPerFect Transfection Reagent are available at the TransFect Protocol Database. (qiagen.com)
A range of suspension cell lines and differentiated and undifferentiated macrophages have been tested using HiPerFect Reagent for siRNA transfection. (qiagen.com)
Transfection with HiPerFect Reagent is a superior alternative to electroporation for many of these cell types. (qiagen.com)
Some cell lines were not amenable to transfection using HiPerFect Reagent (see Table 2). (qiagen.com)

Her work was focused on thiol biochemistry, uncovering targets of the neutrophil-derived oxidant hypochlorous acid in endothelial cells. (otago.ac.nz)
Mast cell degranulation also induces activation of vascular endothelial cells and expression of chemokines and adhesion molecules ( Ono and Abelson, 2005 ). (frontiersin.org)

Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. (wikipedia.org)
The Jurkat cell line (originally called JM) was established in the mid-1970s from the peripheral blood of a 14-year-old boy with T cell leukemia. (wikipedia.org)
Jurkat J6 cells have been found to produce a xenotropic murine leukemia virus (X-MLV) (referred to as XMRV) that could potentially affect experimental outcomes. (wikipedia.org)
Here, we assessed how common cancer chemotherapeutic agents perturb cytosine methylation in Jurkat-T acute lymphoblastic leukemia cells. (nih.gov)
Background CAR T-cells have exhibited efficacious treatment of hematological malignancies, such as Acute Myeloid Leukemia (AML). (bmj.com)
Here we develop and utilize a capture-based sequencing panel, which covers host hotspot genes and the full-length genome of human T-cell leukemia virus type-1 (HTLV-1), to investigate the clonal architecture of adult T-cell leukemia-lymphoma (ATL). (nature.com)
Adult T cell leukemia-lymphoma (ATL) is an aggressive T cell malignancy caused by the human T cell leukemia virus type 1 (HTLV-1). (nature.com)
Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. (exbio.cz)
Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. (exbio.cz)
Genetic features of precursor B-cell phenotype Burkitt leukemia with IGH-MYC rearrangement. (cdc.gov)
Investigation the Cytotoxicity of 5-AZA on Acute Lymphoblastic Leukemia Cell Line In Vitro and Characterization the Underlying Molecular Mechanisms of Cell Death and Motility. (cdc.gov)

Inhibition of cell growth. (medscape.com)
Studies with murine knock out cells revealed that silymarin inhibition of both mTOR and NF-κB was partially AMPK dependent, whereas silymarin inhibition of mTOR required DDIT4. (nih.gov)

For example, in a single tube the technique allows for the ready detection and characterization of the major cell subsets in blood or bone marrow. (nih.gov)
9. Expansion and characterization of cancer stem-like cells in squamous cell carcinoma of the head and neck. (nih.gov)

In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. (thermofisher.com)
The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells. (exbio.cz)

Methods: We present whole-transcriptome RNA-Sequencing data for Jurkat E6.1 cells in the resting state and two hours post-activation via TCR and CD28. (ox.ac.uk)
We compare early transcriptional responses in the presence and absence of the chemokines CXCL12 and CCL19, and perform a basic comparison between observed transcriptional responses in Jurkat E6.1 cells and those in primary human T cells using publicly deposited data. (ox.ac.uk)
Results: Jurkat E6.1 cells have many of the hallmarks of standard T cell transcriptional responses to activation, but lack most of the depth of responses in primary cells. (ox.ac.uk)
Conclusions: These data indicate that Jurkat E6.1 cells hence represent only a highly simplified model of early T cell transcriptional responses. (ox.ac.uk)

2023 ACE2 expressing HEK293 cells Bianchini F. et al. (invivogen.com)

Jurkat and human normal prostate epithelial cells were treated with benzo[a]pyrene to ascertain the epigenetic effects of this type of agent. (cdc.gov)

Methods Lentiviral transduction of two fluorescent proteins (mOrange2 and mTurquoise2) under either the constitutively active EF1alpha (FP1) or the NFAT-sensitive minimal IL-2 promoter (FP2) into Jurkat cells resulted in two IRS cell lines. (bmj.com)
Consistent with Vpr's ability to impair cell-cycle progression, we observed Vpr-mediated modulation of spindle and centromere proteins, as well as Aurora kinase A and cyclin-dependent kinase 4 (CDK4). (nih.gov)
They are formed from the binding of specific proteins on the surface of the joined cells. (scienceblogs.com)
These proteins travel and are organized on the surface of the cell using the cytoskeleton. (scienceblogs.com)
The expression of cell cycle-related proteins cyclin B1, CDC2 and apoptosis-related protein Bcl-2 was evaluated by Western blot assay. (bvsalud.org)

Upon antigen binding, CARs initiate Ca2+-dependent signaling pathways, leading to an increased intracellular concentration of nuclear factor of activated T cells (NFAT), which stimulates downstream T cell effector functions. (bmj.com)
Here, we report on a CAR screening platform that utilizes an NFAT-sensitive promoter driving fluorescent protein expression in Jurkat T cells. (bmj.com)
Calcium-NFAT transcriptional signaling in T cell activation and T cell exhaustion. (bmj.com)
Dose response of CD20 Ab in ADCC Bioassay Effector Cell (Low Affinity/NFAT-Jurkat cells). (cobioer.com)
2023 Jurkat-Lucia™ NFAT Cells for ADCC & ADCP Assays Hederman A.P. et al. (invivogen.com)

Nanosecond electric pulses cause mitochondrial membrane permeabilization in Jurkat cells med. (emf-portal.org)
A stable THP-1 cell line overexpressing SR-F1 was generated and C1q was shown to bind more strongly to the surface of SR-F1 overexpressing macrophages, with C1q/SR-F1 colocalization observed in some membrane areas. (frontiersin.org)
Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism involving an effector cell lysing a target cell on which antibodies have bound to specific antigens on the target cell membrane. (cobioer.com)
The recipient cell is enveloping the virus with its membrane and pulling those vesicles into itself -- a process called endocytosis. (scienceblogs.com)

Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell culture banks. (wikipedia.org)
The I 2.1 cell line is functionally defective for FADD and the I 9.2 cell line is functionally defective for caspase-8, both defective molecules being essential to apoptosis or necroptosis of cells. (wikipedia.org)
It is surprising that we evidenced PBR protein expression in this cell line by means of Western blotting and immunocytochemistry assays using specific anti-PBR antibodies. (unipi.it)
They are possibly the best-known T-cell line making them a significant cell line. (oxinst.com)
However, in recent years some caution has been advised when using this cell line in modelling typical T-cell function since a number of mutations have been identified. (oxinst.com)
Nevertheless, it is a highly valuable cell line for these, and many other studies. (oxinst.com)
Most notably this cell line has been very useful for study of HIV, the causative virus of AIDS. (oxinst.com)
It is complexities like this that can occur within clinical cases that can be difficult, if not impossible to replicate in cell line models. (oxinst.com)
32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested. (cobioer.com)
14. The CD133+ subpopulation of the SW982 human synovial sarcoma cell line exhibits cancer stem-like characteristics. (nih.gov)

described a homophilic antibody that induced apoptosis in human tumor cells without the help of immune effector cells. (medscape.com)

Scientific topics are molecular and cell biological aspects of tumor cell resistance, characterisation of RNA and protein expression patterns and molecular analysis of chromosomal translocations to better understand development and progression of malignancies. (studserv.de)

Furthermore, clonally expanded, premalignant cells harboring putative driver mutations were detected in the peripheral blood of individuals at high risk for ATL before clinical onset, indicating that the development of ATL involves the progressive accumulation of mutations within an infected T cell clone 12 . (nature.com)

The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. (biolegend.com)

The typical ADCC involves activation of natural killer (NK) cells by antibodies. (cobioer.com)

Elevated expression of CD47 in some cancers is associated with poor survival related to its function as an innate immune checkpoint when expressed on tumor cells. (nih.gov)
T2611ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. (topsan.org)
T2610ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. (topsan.org)
T2609ZtA6 cells are derived from spontaneous tumor-like hypertropheied testis isolated from albino-type (alb-1/alb-1) zebrafish. (topsan.org)
10. Coexpression of CD44-positive/CD133-positive cancer stem cells and CD204-positive tumor-associated macrophages is a predictor of survival in pancreatic ductal adenocarcinoma. (nih.gov)
13. Expression of cancer stem cell markers ALDH1, CD44 and CD133 in primary tumor and lymph node metastasis of gastric cancer. (nih.gov)

Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation. (wikipedia.org)
Jurkat cells are used most significantly to study T-cell Leukaemia, HIV and certain cancers. (oxinst.com)

Aberrant cyclization affords a C-6 modified cyclic adenosine 5'-diphosphoribose analogue with biological activity in Jurkat T cells. (ox.ac.uk)
In the present study, we investigated the biological role and potential of miR-338-3p in MM. We found that miR-338-3p was significantly decreased in newly diagnosed and relapsed MM tissues and cell lines. (techscience.com)

FP2 expression, driven only in activated cells, represents antigen recognition by and activity of the CAR. (bmj.com)
Importantly, the use of IRS cells is not restricted to just CD33CARs but provides a platform for CAR and recombinant TCR screenings against any antigen. (bmj.com)
This binding results in the activation have been implicated with streptococcal toxic shock syn- of a large proportion of antigen-presenting cells and T drome (STSS). (cdc.gov)

Pyo, CW, Lee, SH & Choi, SY 2008, ' Oxidative stress induces PKR-dependent apoptosis via IFN-γ activation signaling in Jurkat T cells ', Biochemical and biophysical research communications , vol. 377, no. 3, pp. 1001-1006. (korea.ac.kr)

The trypan blue dye exclusion assay is one of the most commonly used assays for evaluating cell viability. (nist.gov)
Here, we seek t o improve the accuracy and precision of trypan blue cell viability assays by implementing control materials to benchmark image quality (e.g. focus and brightness). (nist.gov)
Figure 3: The use of control beads as an in-measurement-process control to verify or achieve imaging at a reference focal plane improves accuracy and reproducibility of trypan blue based cell viability assays. (nist.gov)

The encoded protein may also play a role in the susceptibility of certain CD4 memory T cells to human immunodeficiency virus infection. (nih.gov)

What are Jurkat Cells used for in cell biology studies? (oxinst.com)

The mechanisms by which human immunodeficiency virus (HIV) circumvents and coopts cellular machinery to replicate and persist in cells are not fully understood. (nih.gov)
The Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. (abcam.com)

Conclusions Our results indicate that IRS cells can be used as an objective, fast, and reliable reporter system for CD33CAR activity. (bmj.com)

The I 9.2 and I 2.1 cell lines. (wikipedia.org)
Peak expression occurred at 12-15 hours post-treatment and consistent, high FP2 signal was detected for 24h in both cell lines. (bmj.com)
Normalized FP2 expression was comparable between both IRS cell lines but varied across the eight CD33CARs. (bmj.com)
Jurkat cell lines can also be used in screening of different antiretroviral treatment strategies. (oxinst.com)
To define how this occurs, transcriptional profiling, metabolomics, and signaling studies were performed in human liver and T cell lines. (nih.gov)
and to Jurkat and HL-60 cell lines grown in suspension. (sigmaaldrich.com)
1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
As experts in innate immunity and cell culture, we offer a large selection of products including PRR ligands , cell lines , selective antibiotics , antimicrobial agents , vaccine adjuvants , and antibodies . (invivogen.com)

This makes Jurkat cells a good model to study some (but not all) of the complexities that are seen in HIV infections. (oxinst.com)

Jurkat cells were untreated (left) or treated (right) with 20 µM Etoposide for 4 hrs before performing Comet Assay (alkaline electrophoresis conditions, 33 V/300 mA for 15 mins). (abcam.com)

Human T-lymphoma Jurkat cells have been considered as lacking PBR and are often used as negative control to prove the specificity of PBR ligands effects. (unipi.it)

Increasing SR-F1 negatively modulated the uptake of apoptotic cells. (frontiersin.org)
Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. (biolegend.com)

To clarify the desialylation mechanism, we studied α2,3-sialyltransferase mRNA expression and the activity of slalidase on the cell surface during etoposide-induced apoptosis. (elsevierpure.com)

This approach, termed 'IL-2 Reporter System' (IRS), is employed for the identification of functional CD33CARs to improve AML CAR T-cell therapies. (bmj.com)
11. [The cultivation and identification of lacrimal gland adenoid cystic cancer stem cells]. (nih.gov)

The ocular allergic response results from exposure of the conjunctiva to an environmental allergen with subsequent binding of specific immunoglobulin E molecules onto conjunctival mast cells. (frontiersin.org)
Blood cells, molecules & diseases 2022 4 95 102662. (cdc.gov)

19. Differential characteristics of CD133(+) and CD133 (-) Jurkat cells. (nih.gov)

Finally, siRNA-mediated depletion of IFN-γ or pkr efficiently downregulated H 2 O 2 -mediated apoptotic cell death. (korea.ac.kr)
Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. (thermofisher.com)

Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. (thermofisher.com)
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/p35/p10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. (ptglab.com)

Cell Biochem Funct, 2021 Jul. (nih.gov)
Cancer cell 2021 1 39 (1): 83-95.e4. (cdc.gov)

Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro med. (emf-portal.org)

2 × 10 5 Jurkat cells grown in presence or absence of antibody. (medscape.com)

IRS cell analysis was performed using flow cytometry, fluorescent microscopy, and IncuCyte live cell imaging. (bmj.com)
Flow cytometry analysis 24hr post treatment determined that 60-80% of transduced IRS cells expressed FP2. (bmj.com)

These Fc receptors recognize and bind to the Fc portion of an antibody, such as IgG, which has bound to the surface of a pathogen-infected target cell. (cobioer.com)

By utilizing a novel, inducible HIV-1 CD4+ T-cell model system expressing either the wild type or a vpr- negative viral genome, we overcame challenges associated with synchronization and infection-levels present in other models. (nih.gov)
The large range of a dedicated QIAsymphony kits enables optimized purification of genomic DNA, cell-free circulating DNA, RNA, bacterial and viral nucleic acids from a wide range of starting materials. (qiagen.com)
However, cell-to-cell transfer of HIV is up to a thousand times more efficient and inhibiting it inhibits viral replication. (scienceblogs.com)

IRS cell function was confirmed by treatment with phorbol myristate acetate (PMA) and ionomycin to induce activation and thus the expression of FP2. (bmj.com)
Results As expected, live cell fluorescence imaging revealed that PMA/ionomycin treatment induced expression of FP2. (bmj.com)
The treatment of cells with the specific JAK-STAT inhibitor, AG490, reduced the PKR expression, and suppressed PKR-dependent cell death. (korea.ac.kr)
The expression of hST3Gal III and hST3Gal IV mRNAs were down-regulated and sialidase activity on the cell surface increased threefold within 2 h of etoposide treatment, Moreover, the decrease in α2,3-linked sialic acid levels was significantly suppressed in the presence of 2,3-dehydro-2-deoxy-H-acetylneuraminic acid, an inhibitor of sialidase. (elsevierpure.com)
These results suggested that activation or exposure of sialidase on the cell surface was induced by etoposide treatment and was the main cause of the decrease in sialic acids. (elsevierpure.com)
Non-viable Jurkat cells were generated via heat shock treatment (70 ° C for 30 min) and spiked into nominally viable cells to achieve cell samples at systematically varied % viabilities. (nist.gov)
Etoposide Treatment of Jurkat Cells. (abcam.com)
Ophthalmic anti-allergic treatment includes topical mast cell stabilizers, oral and topical antihistamines, antihistamine-vasoconstrictor combinations, dual action agents with mast cell-stabilizing, and antihistaminic properties, and anti-inflammatory agents including steroids and non-steroidal drugs. (frontiersin.org)

A cell suspension is mixed with the dye and then immediately visualized under brightfield microscopy (manually or automated) to determine whether cells take up or exclude the dye. (nist.gov)

Anatomy15

Organisms3

Diseases4

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes21

Information Science3

Lysate5

Apoptosis in Jurkat T cells1

Receptor17

Gene8

HeLa3

Proliferation6

Transfection5

Endothelial cells2

Leukemia11

Inhibition2

Characterization2

Reacts2

E6.14

HEK2931

Epithelial1

Proteins5

NFAT5

Membrane4

Line10

Effector1

Protein expression1

Peripheral1

Interactions1

ADCC1

Tumor6

Cancers2

Biological2

Antigen3

Induces1

Assays3

Susceptibility1

Biology1

Cellular2

Conclusions1

Lines8

Infections1

Assay1

Ligands1

Negatively2

Etoposide induced apoptosis1

Identification2

Molecules2

Differential1

Apoptotic2

Caspase2

20212

Vitro1

Grown1

Flow cytometry2

Antibody1

Viral3

Treatment8

Suspension1