• In T cells, ZAP-70 phosphorylation is dependent on lymphocyte-specific protein tyrosine kinase (Lck) activity, which in turn is either positively or negatively regulated by the phosphorylation of specific Lck tyrosine residues. (nih.gov)
  • In particular, the general belief is that Lck is negatively regulated by phosphorylation of tyrosine 192 (Y192). (nih.gov)
  • 22. CD45 modulates T cell receptor/CD3-induced activation of human thymocytes via regulation of tyrosine phosphorylation. (nih.gov)
  • 24. Negative signaling in B lymphocytes induces tyrosine phosphorylation of the 145-kDa inositol polyphosphate 5-phosphatase, SHIP. (nih.gov)
  • 32. gp120 ligation of CD4 induces p56lck activation and TCR desensitization independent of TCR tyrosine phosphorylation. (nih.gov)
  • Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors. (lookformedical.com)
  • They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES , which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES). (lookformedical.com)
  • Activation of T lymphocytes through their TCR is regulated by a delicate balance of phosphorylation and dephosphorylation of protein substrates by protein tyrosine kinases (PTKs) and phosphotyrosyl phosphatases, respectively. (uaeu.ac.ae)
  • In this report, we demonstrate that specific inhibition of Lck expression in Th2 cells, in the presence of normal levels of functional Fyn PTK, has profound consequences on multiple events following TCR stimulation, including an altered pattern of tyrosine-phosphorylated substrates, defective phosphorylation of TCR-ζ and ZAP-70, defective Ca 2+ mobilization, and a ∼90% reduction in proliferate responses to antigenic and mitogenic stimuli. (uaeu.ac.ae)
  • CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. (exbio.cz)
  • Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. (cusabio.com)
  • T-cell receptor signaling is mediated by transient Lck activity, which is inhibited by inorganic mercury. (nih.gov)
  • 25. Both T cell receptor (TcR)-CD3 complex and CD2 increase the tyrosine kinase activity of p56lck. (nih.gov)
  • 26. The coreceptor CD4 is expressed in distinct nanoclusters and does not colocalize with T-cell receptor and active protein tyrosine kinase p56lck. (nih.gov)
  • 33. CD4-dependent and -independent association of protein tyrosine kinases to the T cell receptor/CD3 complex of CD4+ mouse T lymphocytes. (nih.gov)
  • Esta enzima es una tirosina cinasa de la familia src específica de los linfocitos, crítica para el desarrollo y activación de las células T. Lck está asociada a los dominios citoplasmáticos de CD4, CD8 y cadena beta del receptor de IL-2. (bvsalud.org)
  • Lck is associated with the cytoplasmic domains of CD4, CD8 and the beta-chain of the IL-2 receptor, and is thought to be involved in the earliest steps of TCR-mediated T-cell activation. (bvsalud.org)
  • Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. (lookformedical.com)
  • In X-linked SCID, loss of IL-2 receptor (IL-2R) function leads to the loss of a lymphocyte proliferation signal. (medscape.com)
  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (cusabio.com)
  • Nintedanib is a potent small molecule inhibitor of the receptor tyrosine kinases PDGF receptor, FGF receptor and vascular endothelial growth factor receptor. (ersjournals.com)
  • Both these receptors are pro-angiogenic receptor tyrosine kinases and nintedanib was designed as an anti-angiogenic drug for cancer indications. (ersjournals.com)
  • The roles of the main target receptor tyrosine kinases of nintedanib in IPF are explained. (ersjournals.com)
  • Cell surface protein-tyrosine kinase receptors for HEPATOCYTE GROWTH FACTOR. (umassmed.edu)
  • 36. The down-modulation of CD4 induced by the GM1 ganglioside is regulated by phosphatases and kinases: evidence from enzyme inhibitors and anti-CD45 antibodies. (nih.gov)
  • We have found this to be the result of an inadequate activation of the tyrosine kinase ZAP-70, which is hypophosphorylated following TCR stimulation in Hg(2+) burdened cells when compared to untreated controls. (nih.gov)
  • 21. Activation of naïve CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling. (nih.gov)
  • CD2 can mediate TcR-CD3-independent and CD45-dependent activation of p56lck. (nih.gov)
  • This enzyme is a lymphoid-specific src family tyrosine kinase that is critical for T-cell development and activation. (bvsalud.org)
  • CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. (thermofisher.com)
  • for example, protein kinase activation is part of the platelet activation signal pathway. (lookformedical.com)
  • Could be a T-cell-specific adapter protein involved in the control of T-cell activation. (nih.gov)
  • A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. (nih.gov)
  • In contrast, Lck-deficient cells expressed constitutively elevated levels of lymphokine mRNA, including IL-4, IL-5, and IL-10, and were capable of secreting IL-4 upon activation through the TCR. (uaeu.ac.ae)
  • One strategy used to specify motor function is usually illustrated by the kinesins which constitute a large and diverse family of proteins that have unique motor properties (Hirokawa homologue of Arfaptin2 ((and during synapse growth we find that Arfaptin (Arfip) also biochemically interacts with dynactin complexes in flies and mice demonstrating a conserved biochemical conversation. (morainetownshipdems.org)
  • Peripheral T cells in mice lacking p56lck do not express significant antiviral effector functions. (ox.ac.uk)
  • Mutant mice lacking p56lck, the T cell-specific protein tyrosine kinase, have a profound thymic atrophy and possess only an immature thymocyte population. (ox.ac.uk)
  • This study evaluated the capacity of these mice to generate antiviral immune responses: 1) mutant mice failed to generate virus-specific CTL after acute infection with lymphocytic choriomeningitis virus (LCMV) or vaccinia virus. (ox.ac.uk)
  • 6) VSV infection of mutant mice elicited normal, specific T cell-independent IgM responses, but no significant switch to IgG. (ox.ac.uk)
  • Non-specific staining has not been observed in BALB/c or C57BL/6 mice. (thermofisher.com)
  • 39. Interactions between the tyrosine kinases p56lck, p59fyn and p50csk in CD4 signaling in T cells. (nih.gov)
  • The dot-immunoassay has been adapted for rapid detection and partial characterization of glycosylphosphatidylinositol (GPI)-linked, transmembrane, and intracellular proteins in Triton X-100 (TX-100) extracts of lymphoma cells and intestinal tissue. (unige.ch)
  • The type II transmembrane protein CD26 and the intracellular tyrosine kinase p56lck partially cofractionated with GPI-linked glycoproteins, and the extent to which they partition into GPI-rich plasma membrane domains could be evaluated. (unige.ch)
  • This antibody clone to Dlg has been reported to immunoprecipitate and weakly recognize PSD-95 on rat cerebrum lysates, presumably due to crossreactivity between homologous regions of the two proteins (i.e amino acid regions 104-119 and 193-198). (bdbiosciences.com)
  • Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
  • Separation of human monocytes (red-filled) from CD31 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD31 (MEM-05) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
  • 40. MHC class I ligation of human T cells activates the ZAP70 and p56lck tyrosine kinases, leads to an alternative phenotype of the TCR/CD3 zeta-chain, and induces apoptosis. (nih.gov)
  • However, p62 has homology neither with ubiquitin C-terminal hydrolases nor with the S5a subunit of the 26 S proteasome complex, the only proteins known to bind to ubiquitin noncovalently. (nih.gov)
  • Also, Dlg has been reported to bind p56 [lck] tyrosine kinase and the Kv1.3 channel in human T lymphocytes. (bdbiosciences.com)
  • Could be an adapter protein that facilitates and regulates interaction of KDR with effector proteins important to endothelial cell survival and proliferation. (nih.gov)
  • These molecular defects interfere with lymphocyte development and function, blocking the differentiation and proliferation of T cells and, in some types, of B cells and NK cells. (medscape.com)
  • Although the pathogenic pathways involved in IPF have not been fully elucidated, IPF is believed to be caused by repetitive alveolar epithelial cell injury and dysregulated repair, in which there is uncontrolled proliferation of lung fibroblasts and differentiation of fibroblasts into myofibroblasts, which excessively deposit extracellular matrix (ECM) proteins in the interstitial space. (ersjournals.com)
  • Innate Conformational Dynamics Drive Binding Specificity in Anti-Apoptotic Proteins Mcl-1 and Bcl-2. (uchicago.edu)
  • These results demonstrate a dissociation in functional responses in Lck-deficient Th2 cells and suggest a role for Lck in the induction of a state of T cell unresponsiveness. (uaeu.ac.ae)
  • A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. (thermofisher.com)
  • We have used three criteria, namely flotation to light density fractions in sucrose gradients, colocalization with GM1, and sensitivity to PI-PLC cleavage, to assess the presence of a GPI modification in a putative GPI-linked protein in intestinal tissue extract. (unige.ch)
  • To study the role of Lck in mature T cells, antisense RNA was used to inhibit its expression in a nontransformed Th2 clone. (uaeu.ac.ae)
  • 35. p56lck association with CD4 is required for the interaction between CD4 and the TCR/CD3 complex and for optimal antigen stimulation. (nih.gov)
  • INTRODUCTION Dynamic cellular processes such as membrane trafficking are predicted to use the precise spatial and temporal regulation of a vast array of proteins including motor proteins such as kinesin and dynein. (morainetownshipdems.org)
  • The mechanisms underlying the precise regulation of motor protein function in neurons are incompletely described. (morainetownshipdems.org)
  • The cytoplasmic portion contains the catalytic domain and sites critical for the regulation of kinase activity. (umassmed.edu)
  • Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. (lookformedical.com)
  • Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments. (lookformedical.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • It interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. (wikipedia.org)
  • CD2 is a specific marker for T cells and NK cells, and can therefore be used in immunohistochemistry to identify the presence of such cells in tissue sections. (wikipedia.org)
  • Thus, it appears that selected T cells can mature without lck and that they may have circumvented the need for this enzyme. (ox.ac.uk)
  • Cells in the lymphocyte gate were used in the analysis. (thermofisher.com)
  • Mechanistically Arfip is usually a membrane-binding protein localized at the Golgi that is required for binding of the dynactin complex to membranes in the motor neuron. (morainetownshipdems.org)
  • The GPI-anchored proteins tend to concentrate into specialized plasma membrane domains enriched in glycosphingolipids. (unige.ch)
  • The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. (uchicago.edu)
  • The human homologue of the Drosophila discs large tumor suppressor protein (hDlg) is a member of the MAGUKs (membrane associated guanylate kinases) protein family. (bdbiosciences.com)
  • They function in the cell cycle to maintain MATURATION PROMOTING FACTOR in the active state and have protein-serine/threonine kinase activity. (uams.edu)
  • Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain. (nih.gov)
  • In DKD, the accumulation of advanced glycation end products induces decreased renal autophagy-related protein expression and transcription factor EB (TFEB) nuclear transfer, leading to impaired autophagy and lysosomal function and blockage of autophagic flux. (bvsalud.org)
  • Thus, Hg(2+) inhibits a critical activating role played by Lck Y192 during the most proximal events of the TCR-induced cell signaling. (nih.gov)
  • May play a role in the CD4-p56-LCK-dependent signal transduction pathway. (nih.gov)
  • Network-based systems pharmacology reveals heterogeneity in LCK and BCL2 signaling and therapeutic sensitivity of T-cell acute lymphoblastic leukemia. (uchicago.edu)
  • Therefore, these data indicate that p56lck is directly or indirectly crucially involved in in vivo Ag-induced T cell responses. (ox.ac.uk)
  • The dot-immunoassay has been successfully used to demonstrate the differential distribution of GPI-linked and transmembrane surface glycoproteins of T lymphocytes in sucrose density gradient fractions of TX-100 lysate. (unige.ch)
  • Treatment of whole cell TX-100 lysates or sucrose gradient fractions dotted onto nitrocellulose filter strips with bacterial phosphatidylinositol-specific phospholipase C (PI-PLC) proved to be an efficient method to assay for the presence of a GPI-anchor in Thy-1 and Ly6 surface glycoproteins. (unige.ch)
  • Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)
  • Patients with a high expression of CD45 had a better local recurrence-free survival and disease-specific survival. (cusabio.com)