Mice knockout. Medical search. Frequent questions

You can quickly query the required genetically engineered global knockout (KO) mouse, conditional knockout (cKO/floxed) mouse and microRNA KO mouse strains by simply entering the gene name. (cyagen.com)
6 Facts You May Not Know About Cre-Lox The basics of conditional knockout mice are familiar to any researcher who. (genetargeting.com)
In order to determine the physiological function of GSKIP, in particular its involvement in PKA and GSK3β signalling, a conditional knockout (KO) mouse model had been developed. (fu-berlin.de)

For addressing the in vivo role of TRPV5 and calbindin-D 28K in the maintenance of the Ca 2+ balance, single- and double-knockout mice of TRPV5 and calbindin-D 28K (TRPV5 -/- , calbindin-D 28K -/- , and TRPV5 -/- /calbindin-D 28K -/- ) were characterized. (researchwithrutgers.com)
To directly assess the role of nNOS in mdz muscle, we generated dystrophin-nNOS double knockout mice by mating nNOS single knockout mice with mdx mice. (johnshopkins.edu)
The double knockout mice are indistinguishable from the rndx mice in terms of dystrophic muscle morphology and sarcolemma membrane damage. (johnshopkins.edu)

The phenotype of the L1 mutant mouse indicates that it is a potentially valuable model for the human CRASH syndrome. (jneurosci.org)
This BDNF pro-domain KO mouse model showed significant weight loss, impaired righting reflex, abnormal motor behaviours and short lifespan (less than 22 days), mimicking a Huntington's disease (HD)-like phenotype. (ozgene.com)
AKR1D1 knockout mice develop a sex-dependent metabolic phenotype. (ox.ac.uk)
Akr1d1-/- mice had a sex-specific metabolic phenotype. (ox.ac.uk)
The AGAT-knockout phenotype of calcium handling was fully reversed by dietary creatine supplementation throughout life. (ox.ac.uk)
A 100 % penetrant phenotype in GSKIP KO mice is the incomplete closure of the palatal shelves. (fu-berlin.de)

There are several thousand different strains of knockout mice. (wikipedia.org)
The Cyagen Knockout Catalog Models repository provides a searchable listing of over 16k mouse strains in more than 20 research fields, including oncology, cardiology, and neurology. (cyagen.com)
By combining high throughput electroporation with gene knockout strategies generated by AlphaKnockout , our proprietary AI (artificial intelligence) gene targeting expert system, and special ovulation induction technologies, we successfully established and cryopreserved 100 strains of CRISPR-AI knockout mouse sperms. (cyagen.com)
Encouraged by the success of the first 100 cases, we decided to create the CRISPR-AI knockout mouse sperm bank with the goal to cryopreserve strains covering most mouse genes. (cyagen.com)
In just a year and a half, we have successfully established and cryopreserved over a thousand custom KO strains from which live mice can be produced in as little as seven weeks. (cyagen.com)
This innovative mouse sperm cryorecovery technology builds on experience accumulated over the past years and we expect to reach 10,000 strains by the end of 2018. (cyagen.com)
These mice strains were fed two Ca 2+ diets (0.02 and 2% wt/wt) to investigate the influence of dietary Ca 2+ content on the Ca 2+ balance. (researchwithrutgers.com)

White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene. (wikipedia.org)
A minimum of four (4) mutant mice, heterozygous for the selected mutation on a mixed 129S5;B6 background. (taconic.com)
T6080The Stable Bcl-x Knockout Mouse Embryonic Fibroblast Cell Line was generated from embryos of a pregnant mouse heterozygous for. (topsan.org)
Heterozygous (Het) mice showed impaired learning and memory capability and depressive-like behaviours, compared with wild type (WT) mice. (ozgene.com)

Here, to confirm that cPKC activity is required for contraction-stimulated glucose uptake in mouse muscles, contraction-stimulated glucose uptake ex vivo was first evaluated in the presence of three commonly used cPKC inhibitors (calphostin C, G{\'o}-6976, and G{\'o}-6983) in incubated mouse soleus and extensor digitorum longus (EDL) muscles. (ku.dk)
However, in muscles from PKCalpha knockout (KO) mice, neither contraction- nor phorbol ester-stimulated glucose uptake ex vivo differed compared with the wild type. (ku.dk)
Here, we explored the role of NPC1L1 in colorectal tumorigenesis in vivo by using transgenic mice. (biomedcentral.com)
In vivo, cardiac-gated, black-blood, and ex vivo magnetic resonance microscopy (MRM) images of the aortic root, and histopathology data were obtained from 12 transgenic and wild-type (WT) mice. (mssm.edu)

Given the expression of IRAP in areas of the brain associated with oxytocin modulated maternal behavior, we also investigated whether the IRAP global knockout mice had improved maternal responses. (monash.edu)

Akr1d1-/- mice were generated on a C57BL/6 background. (ox.ac.uk)
Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. (cdc.gov)
B6.129X1-MPO (MPO k/o) vs wild-type C57Bl/6 mice (w/t) and induction of mesotheliomas) [8,9]. (cdc.gov)

Gene knockout in rats is much harder and has only been possible since 2003. (wikipedia.org)
Inducible Control of Your Tissue-Specific Animal Model The Cre-lox system is commonly used to provide tissue-specific gene knockout. (genetargeting.com)
AlphaKnockout is a gene targeting expert system developed by Cyagen Biosciences for designing gene knockout strategies based on artificial intelligence (AI). (cyagen.com)
Supported by the powerful processing capacity of Tianhe-2 supercomputer, it can generate and display gene knockout strategies in as little as 10 seconds. (cyagen.com)

T6303To generate the COX10 Stable Knockout Mouse Fibroblast Cell Line, a primary skin fibroblast culture was established from a knockin mouse. (topsan.org)

The key difference between transgenic and gene targeted (knockout or knockin) animals is that in knockout and knockin models the genomic changes are targeted, meaning a desired DNA sequence is altered at a specific locus in the target genome via homologous recombination, with an engineered segment of DNA replacing a homologous endogenous segment. (cyagen.com)
Knockout and Knockins are usually made using homologous recombination in ES cells, but nuclease-mediated genome editing using TALEN and CRISPR/Cas9 can also be used to generate knockouts or small sequence changes. (cyagen.com)
To create such knockout animals, labs use the cre-lox system that modulates the genomic DNA through homologous recombination. (pxl.be)

Is It Mice or Rats? (linx.com)
Mice and rats are common in New York homes, apartments, and businesses. (linx.com)
Carcinogenicity and chronic toxicity in rats and mice exposed to chloroform by inhalation. (who.int)

If the guide RNAs were (i) tested in mouse embryos by the core and the core approved their use based on the genotyping results and (ii) the core conducted the founder genotyping, the core can review results, redesign the approach and reagents if needed, and the electroporation can be repeated. (bcm.edu)

A knockout mouse, or knock-out mouse, is a genetically modified mouse (Mus musculus) in which researchers have inactivated, or "knocked out", an existing gene by replacing it or disrupting it with an artificial piece of DNA. (wikipedia.org)
As the leader in custom genetically engineered mouse and rat models, Cyagen has generated thousands of custom knockout, knockin and transgenic rodent lines for its increasing customer base in over 3,000 universities and companies worldwide. (cyagen.com)

To study the mechanisms by which loss of PrP function may underlie changes in vulnerability to epilepsy in disease, we used several acute epilepsy models: we applied a variety of convulsant treatments (zero-magnesium, bicuculline, and pentylenetetrazol) to slices in vitro from PrP knockout (Prnp0/0) and control mice. (ox.ac.uk)
In all three epilepsy models, we found that longer delays and/or higher concentrations of convulsants were necessary to generate spontaneous epileptiform activity in Prnp0/0 mice. (ox.ac.uk)

The present study explores the possible involvement of a purinergic mechanism in mechanosensory transduction in the bladder using P2X 3 receptor knock-out (P2X 3 − /− ) and wild-type control (P2X 3 +/+ ) mice. (jneurosci.org)
We found group-housed Grb10 +/p mice do not show evidence of enhanced social dominance, but cages containing Grb10 +/p and wild-type mice lacked the normal correlation between three different measures of social rank. (bath.ac.uk)
Nicotine responses in hypersensitive and knockout alpha 4 mice account for tolerance to both hypothermia and locomotor suppression in wild-type mice. (umassmed.edu)
Wild-type mice and NPC1L1 −/− (NPC1L1 knockout) mice were treated with azoxymethane (AOM)-dextran sodium sulfate (DSS) to induce colitis-associated colorectal tumorigenesis. (biomedcentral.com)
NPC1L1 −/− mice significantly had fewer tumors than wild-type. (biomedcentral.com)
The ratio of malignant/tumor in NPC1L1 −/− mice was significantly lower than in wild-type 20 weeks after AOM-DSS treatment. (biomedcentral.com)
NPC1L1 was highly expressed in the small intestine of wild-type mice but its expression was undetectable in colorectal mucous membranes or tumors in either group. (biomedcentral.com)
NPC1L1 −/− mice had significant lower intestinal inflammation scores and expressed inflammatory markers p-c-Jun, p-ERK and Caspase-1 p20 lower than wild-type. (biomedcentral.com)
We studied how the absence of a functional CK system influences calcium handling in isolated cardiomyocytes from AGAT-knockouts and wild-type littermates as well as in AGAT-knockout mice receiving lifelong creatine supplementation via the food. (ox.ac.uk)
Using a combination of whole cell patch clamp and fluorescence microscopy, we demonstrate that the L-type calcium channel (LTCC) current amplitude and voltage range of activation were significantly lower in AGAT-knockout compared with wild-type littermates. (ox.ac.uk)
According to our modeling results, these changes can be reproduced by reducing three parameters in knockout mice when compared with wild-type: LTCC conductance, the exchange constant of Ca2+ transfer between subspace and cytosol, and SERCA activity. (ox.ac.uk)
Urine analysis indicated that TRPV5 -/- / calbindin-D 28K -/- mice exhibit on both diets hypercalciuria compared with wild-type mice. (researchwithrutgers.com)
Intestinal Ca 2+ absorption and expression of calbindin-D 9K and TRPV6, as well as serum parameters of the calbindin-D 28K -/- mice, did not differ from those of wild-type mice. (researchwithrutgers.com)
Sleep, body temperature (BT), locomotor activity, arterial pressure (AP) and heart rate (HR) were recorded in KO mice and wild-type (WT) controls under ad libitum-fed conditions and 24-hour food deprivation (FD). (tokushima-u.ac.jp)
Mossy fiber LTD in Grm2/3 dko mice was indistinguishable from that in wild-type mice at 4 mM Ca2+, but largely absent at 2 mM external Ca2+. (ox.ac.uk)
Body and lung weights are unchanged compared to wild type mice. (fu-berlin.de)
We have therefore decided to perform a comprehensive study of the substrates of the protease by comparing the 2D electrophoretic profile of myotubes from obtained from calpain-3 knockout and wild type mice. (lu.se)
We could identify 16 proteins which differed in knockout and wild type mice. (lu.se)

A variety of (tissue-specific) Ndufs4 knockout mouse models were developed to study the Leigh syndrome pathomechanism and intervention testing. (nih.gov)
To circumvent embryonic lethality of the conditional GSKIP KO mice, tissue-specific promoters combined with the Cre/loxP-system were used to generate two adult inducible GSKIP KO mouse models. (fu-berlin.de)

Moreover, the original study examined tube-test behavior in isolated mice 10 months of age. (bath.ac.uk)

Threshold for epileptiform activity is elevated in prion knockout mice. (ox.ac.uk)

They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology. (wikipedia.org)
Due to compensatory genetic mechanisms, knockout status cannot be guaranteed and will have to be confirmed by the customer. (taconic.com)
To identify possible genetic interactions between themechanisms of tumor suppression of menin and pRb, weintercrossed mice with targeted deletions of Men1 andRb1, and compared tumor development in cohorts ofanimals carrying single or dual mutations of these tumorsuppressorgenes. (edu.au)

We report here that Ptch +/- mice develop primordial follicular neoplasms resembling human trichoblastomas, and that exposure to ultraviolet radiation or ionizing radiation results in an increase in the number and size of these tumors and a shift in their histologic features so that they more closely resemble human basal cell carcinoma. (nature.com)
The mouse basal cell carcinomas and trichoblastoma-like tumors resemble human basal cell carcinomas in their loss of normal hemidesmosomal components, presence of p53 mutations, frequent loss of the normal remaining Ptch allele, and activation of hedgehog target gene transcription. (nature.com)
The Ptch mutant mice provide the first mouse model, to our knowledge, of ultraviolet and ionizing radiation-induced basal cell carcinoma-like tumors, and also demonstrate that Ptch inactivation and hedgehog target gene activation are essential for basal cell carcinoma tumorigenesis. (nature.com)
NPC1L1 knockout in mice decreased β-catenin in tumors and regulated TGF-β and p-gp in adjacent colons or tumors. (biomedcentral.com)
In mice heterozygousfor both Men1 and Rb1, pancreatic hyperplasiaand tumors of the intermediate pituitary and thyroidoccurred at high frequency. (edu.au)

We assessed the reproductive and maternal profile of global IRAP knock out mice. (monash.edu)
DNAJB6 is an essential protein and knock-out mice die already in uteri due to failure in keratin turnover and placental development. (lu.se)

Many mouse models are named after the gene that has been inactivated. (wikipedia.org)
Other mouse models are named according to their physical characteristics or behaviours. (wikipedia.org)
Here, we review and discuss the role of complex I and NDUFS4 mutations in human mitochondrial disease, and review how the analysis of Ndufs4 knockout mouse models has generated new insights into the MC1ND1/Leigh syndrome pathomechanism and its therapeutic targeting. (nih.gov)
The knockout models are designed to be null alleles. (taconic.com)
As Cyagen Knockout Catalog Models repository has been growing, it is necessary to manually check whether the strain you need is in the recent resource bank construction plan. (cyagen.com)
The powerful database offers you a more convenient experience by streamlining the process of identifying KO mouse models and ordering them for research. (cyagen.com)
Cyagen subdivides different mouse models into applied research fields and provides detailed information about the backgrounds and associated applications of the models to help simplify the model selection process. (cyagen.com)
Cyagen's top priority is to provide high quality mouse and rat models. (cyagen.com)
We offer all the services necessary to create your desired mouse or rat models. (cyagen.com)
Knockout mouse models as a resource for the study of rare diseases. (bvsalud.org)
CNT in biochemical models and in cells yielding the products engineered CNT represent a possible health risk due to their that did not cause pulmonary inflammation in mice [14]. (cdc.gov)
In the presented papers in this thesis we investigated the role of CysLT1R in different mouse models of colorectal cancer. (lu.se)
Also, a decreased nuclear expression of β-catenin within the epithelial tumor compartment was determined for CysLT1R mutant female mice in both models. (lu.se)
In conclusion, the presented mouse models of colorectal cancer further strengthen our previous in vitro findings and highlight the prospect of CysLT1R as an alternative therapeutic approach. (lu.se)
2003]. The absence of STAT5B is associated with diminished post-natal growth, as demonstrated by mouse knockout models [Udy et al. (lu.se)

CRISPR/Cas9-mediated NHEJ in mouse embryos will be used to generate founder animals harboring insertion/deletion (indel) or interval deletion knockout alleles. (bcm.edu)
If the guide RNAs were not tested in mouse embryos or the investigator conducts their own genotyping, there will be no reduced costs for additional work. (bcm.edu)
This includes free strategy design prior to your purchase, construction of transgenic or targeting vectors (including BAC recombineering), creating and characterizing targeted ES cells, microinjection of mouse or rat embryos, and breeding and genotyping of the resulting animals. (cyagen.com)
For enhancer testing in mouse embryos using the LacZ reporter, we also offer LacZ staining of embryos. (cyagen.com)
Building on our proprietary Noncontrolled-Rate Protein-Free Cryopreservation Media, we have developed optimized cryopreservation techniques for mouse sperms and embryos which have greatly improved stability and increased cryorecovery and birth rates. (cyagen.com)
Downregulation of GSK3β Ser9 phosphorylation upon GSKIP KO was observed in mice early in development in whole embryos, E12.5 primary MEFs and also in later stage E18.5 organs, in line with previous observations in cells and validating the KO mouse model. (fu-berlin.de)

However, western blot analysis of pregnant mouse serum, failed to detect IRAP, a result which was confirmed by fluorimetric IRAP enzyme assay. (monash.edu)
Abnormal morphologic changes were found in the dentate gyrus (DG) of the hippocampus in pro-BDNF KO mice, and western blot confirmed significant cell apoptosis in pro-BDNF KO mice brains. (ozgene.com)

P2X 3 -positive staining was completely absent in the subepithelial plexus of the P2X 3 − /− mice, whereas staining for calcitonin gene-related peptide and vanilloid receptor 1 receptors remained. (jneurosci.org)

For example, the p53 knockout mouse is named after the p53 gene which codes for a protein that normally suppresses the growth of tumours by arresting cell division and/or inducing apoptosis. (wikipedia.org)
In situ detection of hedgehog target genes and immunohistology of Ptch +/- mouse BCC differentiation markers, hemidesmosomal proteins and Bcl2 protein. (nature.com)
Homozygous Ambn mutant mice expressing an internally truncated AMBN protein deposit only a soft mineral crust on the surface of dentin. (ozgene.com)
Results: No AMBN protein was detected using immunohistochemistry in null mice. (ozgene.com)

We have inactivated the gene encoding the 5-HT1A receptor in mice and found that receptor-deficient animals have an increased tendency to avoid a novel and fearful environment and to escape a stressful situation, behaviors consistent with an increased anxiety and stress response. (biopsychiatry.com)
To this end, respiration of unrestrained adult and neonatal adenosine A1 receptor knockout mice (A(1)R(-/-)) was measured in a plethysmographic device. (uni-regensburg.de)
In conclusion, these data indicate that the adenosine A(1) receptor is an important molecular component mediating hypoxic depression in adult mice and it appears to stabilize respiration of neonatal mice. (uni-regensburg.de)
Insulin receptor substrate-2-deficient (IRS2-/-) mice are considered a good model to study the development of diabetes because IRS proteins mediate the pleiotropic effects of insulin-like growth factor-I (IGF-I) and insulin on metabolism, mitogenesis and cell survival. (uam.es)

How does Cyagen house its mice? (cyagen.com)

T6304To generate the Rieske ISP Stable Knockout Mouse Fibroblast Cell Line, primary lung fibroblast culture was established from a. (topsan.org)
Genome editing using TALEN or CRISPR/Cas9 can generate a knockout or knockin in as little as ~2 months, far faster than the typical 8-12 months required for conventional knockouts, because there is no need for ES cell work or chimera production. (cyagen.com)

Our results provide the first evidence that NPC1L1 knockout protects against colitis-associated tumorigenesis. (biomedcentral.com)

Because some inflammatory markers are elevated in the hypothalamus of diabetic IRS2-/- mice, our aim was to analyze whether the diabetes associated with the absence of IRS2 results in hypothalamic injury and to analyze the intracellular mechanisms involved. (uam.es)

Creatine-deficient mice, which lack arginine-glycine amidinotransferase (AGAT) to synthesize creatine and homoarginine, exhibit reduced cardiac contractility. (ox.ac.uk)
Our results indicate reduced calcium cycling in cardiomyocytes from AGAT-knockouts and suggest that the creatine kinase system is important for the development of calcium handling in the heart.NEW & NOTEWORTHY Creatine-deficient mice lacking arginine-glycine amidinotransferase exhibit compromised cardiac function. (ox.ac.uk)
GSKIP-deficient mice die at birth, presumably because they are unable to initiate breathing properly. (fu-berlin.de)
2012) Impaired Clearance and Enhanced Pulmonary Inflammatory/Fibrotic Response to Carbon Nanotubes in Myeloperoxidase-Deficient Mice. (cdc.gov)

We also investigated the role of CysLT1R in 1) FAP/sporadic colorectal cancer by crossing ApcMin/+ mice with mice lacking CysLT1R expression and in 2) colitis-associated colorectal cancer by employing the AOM/DSS-model on mice lacking CysLT1R expression. (lu.se)
However, the mechanism of tumor progression in FAP/sporadic colorectal cancer and in colitis-associated colorectal cancer might differ as indicated by reduced tumor expression of COX-2 and reduced serum levels of PGE2 in the female double mutant (CysLT1R−/− ApcMin/+) mice, whereas AOM/DSS-treated female single mutant (CysLT1R−/−) mice demonstrated increased serum levels of PGE2. (lu.se)

Humans share many genes with mice. (wikipedia.org)

In the motor, somatosensory, and visual cortex, many pyramidal neurons in layer V exhibited undulating apical dendrites that did not reach layer I. The hippocampus of L1 mutant mice was smaller than normal, with fewer pyramidal and granule cells. (jneurosci.org)
Enlarged ventricles and septal abnormalities were also features of the mutant mouse brain. (jneurosci.org)

Research has shown that using Inhibitory antiserum (IAS) and conventional super ovulation hormones (PMSG & HCG) greatly increases mouse ovulation. (cyagen.com)

A laboratory mouse in which a gene affecting hair growth has been knocked out (left) is shown next to a normal lab mouse. (wikipedia.org)
Ameloblasts in the AmbnlacZ/lacZ mice were unable to initiate appositional growth and started to degenerate and deposit ectopic mineral. (ozgene.com)
In the mouse xenograft model of colon cancer, we were able to observe a reduced tumor growth in response to CysLT1R antagonist treatment. (lu.se)

In summary, the hypothalamus of diabetic IRS2-/- mice showed an increase in oxidative stress and inflammatory markers that finally resulted in cell death via substantial activation of the extrinsic apoptotic pathway. (uam.es)
Docosahexaenoic acid attenuates hepatic inflammation, oxidative stress, and fibrosis without decreasing hepatosteatosis in a Ldlr(-/-) mouse model of western diet-induced nonalcoholic steatohepatitis. (colorado.edu)

Compared to WT mice, KO mice showed a reduced concentration of plasma ketone bodies and decreased mRNA expression of the ketogenic enzyme gene Hmgcs2 in the liver and brain under FD conditions. (tokushima-u.ac.jp)

Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo) and grown in vitro. (wikipedia.org)

NPC1L1 knockout decreasing plasma lipid, especially cholesterol, to reduce inflammation and decreasing β-catenin, p-c-Jun and p-ERK may be involved in the mechanism. (biomedcentral.com)
At 30 weeks of age, male, but not female, Akr1d1-/- mice were more insulin tolerant and had reduced lipid accumulation in the liver and adipose tissue yet had hypertriglyceridemia and increased intramuscular triacylglycerol. (ox.ac.uk)
These results suggest that PPARα and/or lipid metabolism is involved in the maintenance of wakefulness and locomotor activity during fasting in mice. (tokushima-u.ac.jp)

For most lines, the mouse ES cells used in generating the model are of the 129S5 strain. (taconic.com)
In order to elucidate pro-BDNF's explicit role, we established a pro-BDNF knockout (KO) mouse model. (ozgene.com)
This AI strategy design tool has significantly accelerated the AI Knockout Mouse Model eBank project. (cyagen.com)
Characterization and reversal of synaptic defects in the amygdala in a mouse model of fragile X syndrome. (colorado.edu)

Inhalation exposure of gas-metal arc stainless steel welding fume increased atherosclerotic lesions in apolipoprotein E knockout mice. (cdc.gov)

However, there has been no detailed study of social dominance in Grb10 +/p mice. (bath.ac.uk)

Male Akr1d1-/- mice were not protected against diet-induced obesity and insulin resistance. (ox.ac.uk)

Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
Therefore the absence of IRAP from the circulation in mice, and other species during pregnancy, is due to the inability of a secretase to cleave placental IRAP to produce a soluble form of the enzyme. (monash.edu)

Hepatic and pulmonary microsomal benzene metabolism in CYP2E1 knockout mice. (cdc.gov)

By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (wikipedia.org)
We examined both male and female mice to account for sex differences and tested cohorts aged 2, 6 and 10 months to examine any developments related to age. (bath.ac.uk)
NS-Pten knockout mice show sex- and age-specific differences in ultrasonic vocalizations. (bvsalud.org)

Knockouts are changes that result in the inactivation of the targeted gene, such that the locus makes no gene product, or makes only a nonfunctional gene product. (cyagen.com)

Immunohistochemistry revealed abundant nerve fibers in a suburothelial plexus in the mouse bladder that are immunoreactive to anti-P2X 3 . (jneurosci.org)

If the cut site is in the coding region of a gene, this will result in frameshift mutations downstream of the site, generating a knockout. (cyagen.com)

Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer, obesity, heart disease, diabetes, arthritis, substance abuse, anxiety, aging and Parkinson's disease. (wikipedia.org)

Docosahexaenoic acid attenuates Western diet-induced hepatic fibrosis in Ldlr-/- mice by targeting the TGFβ-Smad3 pathway. (colorado.edu)

Accordingly, the volume of SR tubules localized around the myofibrils is strongly reduced in skeletal muscle fibers of 4- and 10-month-old sAnk1 knockout (KO) mice, while additional structural alterations only develop with aging. (mdpi.com)
To verify whether the lack of sAnk1 also alters intracellular Ca 2+ handling, cytosolic Ca 2+ levels were analyzed in stimulated skeletal muscle fibers from 4- and 10-month-old sAnk1 KO mice. (mdpi.com)
Proteomics revealed the muscle-specific intermediate filament desmin to be at least 50 % downregulated in GSKIP-depleted lungs at E18.5, allowing for a potential connection of mitochondrial aberrations with respiratory failure ultimately causing the perinatal lethality of GSKIP KO mice. (fu-berlin.de)

NPC1L1 knockout also reduced lymphadenectasis what may be caused by inflammation. (biomedcentral.com)

Male Akr1d1-/- mice were challenged with a high fat diet (60% kcal from fat) for 20 weeks. (ox.ac.uk)
CysLT1R knockout female mice, but not male mice exhibited a reduced polyp formation in the small intestine and colon, respectively. (lu.se)

TRPV4 knockout mice at age 8 weeks exhibited normal, but those at 24 weeks revealed significantly higher thresholds by auditory brainstem response. (nih.gov)
The auditory threshold shift was significantly larger in the TRPV4 knockout than in the TRPV4+/+ mice 1 week after the acoustic overexposure of 128dB SPL. (nih.gov)

Anatomy1

Organisms2

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes3

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science2

Health Care1

Conditional knockout3

Double-knockout mice3

Phenotype6

Strains7

Heterozygous4

Vivo4

Global knockout1

C57BL3

Gene knockout4

Knockin mouse1

Homologous recombination3

Rats3

Electroporation1

Genetically2

Epilepsy2

Wild-type18

Tissue-specific2

Behavior1

Prion1

Genetic3

Tumors5

Knock out2

Models15

Embryos6

Western blot2

Receptors1

Protein4

Receptor4

Cyagen1

Generate2

Tumorigenesis1

Mechanisms1

Deficient mice4

Colitis-associated colorectal2

Genes1

Mutant2

Conventional1

Growth3

Oxidative stress2

Brain1

Vitro1

Lipid3

Model4

Lesions1

Social dominance1

Insulin1

Species2

Metabolism1

Differences3

Inactivation1

Immunohistochemistry1

Mutations1

Anxiety1

Hepatic1

Muscle3

Inflammation1

Male2

Auditory2