Activation of alpha 1-adrenoceptors appears to amplify beta-adrenergic stimulation of cyclic AMP (cAMP) accumulation in rat pinealocytes severalfold by a mechanism involving activation of a Ca2+-, phospholipid-dependent protein kinase (protein kinase C). The mechanism of action of protein kinase C was investigated in this report using intact cells. (nih.gov)
Dexamethasone3
The most commonly used synthetic glucocorticoid in adipocyte differentiation procedures tends to be dexamethasone and the most commonly used phosphodiesterase inhibitor is 1-methyl-3-isobutyl-xanthine (IBMX). (wikipedia.org)
To determine which factors are most important for regulation of ob mRNA expression, we examined the effects of insulin, dexamethasone, a β 3 - adrenergic agonist (CGP12177A), 8-bromo-cAMP, 8-bromo-cGMP and 1-methyl-3-isobutylxanthine (MM) on primary cultured adipocytes. (elsevierpure.com)
Furthermore, 954 drugs or compounds were shown to have therapeutic potential for recurrent POP, and the most critical target drugs were dexamethasone, bisphenol A, efavirenz, 1-methyl-3-isobutylxanthine, and estradiol. (bvsalud.org)
Insulin1
Moreover, insulin increased ob mRNA expression in a dose-dependent manner over a range of 1-100 nmol/l. (elsevierpure.com)
Activation1
This stimulation represents up to 40% of the level produced by maximum activation of the arylhydrocarbon receptor (AhR) with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). (nih.gov)
Rapidly1
The cAMP response following cholera toxin pretreatment (60-120 min) was rapidly and markedly enhanced by alpha 1-adrenergic agonists (cirazoline greater than PE greater than methoxamine), by phorbol esters (PMA greater than 4 beta-phorbol 12,13,-dibutyrate much greater than 4 alpha-phorbol 12,13-didecanoate), and by synthetic diacylglycerols (1,2-dioctanoylglycerol greater than 1-oleoyl 2-acetylglycerol much greater than diolein). (nih.gov)
Presence1
The amplification of the beta-adrenergic cAMP response by these agents also occurred in the presence of isobutylmethylxanthine (10(-3) M) and Ro 20-1724 (10(-4) M), an observation suggesting that inhibition of cAMP phosphodiesterase activity is not the mechanism of action. (nih.gov)
Cells3
The 3T3 process began with cultures having 3 days to propagate on a plate (the first "3"), and then a transfer (the "T") of 300,000 cells (second "3") to a new plate to restart the process. (wikipedia.org)
In contrast, seropositive donor cultures seeded at the higher cell concentrations developed foci of proliferating EBNA-positive cells within the first 1--2 weeks but thereafter regressed completely and subcultures made after 4 weeks never gave rise to cell lines. (nih.gov)
For example, Non-Patent Literatures 1 and 2 disclose that importance is placed on the state of cells before freezing, and cells in a logarithmic growth phase are suitable for cryopreservation. (justia.com)
Effect1
EGF exerted a half-maximal reduction in depolarization-stimulated 45Ca2+ uptake at 0.1 nM and a maximal effect at 1-10 nM. (nih.gov)
Response1
EGF treatment increased the amount of PRL secreted basally, but inhibited the acute PRL secretory response to depolarization with 50 mM KCl and 1 microM BAY K8644 from 2.6- to 1.5-fold. (nih.gov)