2'-5' oligoadenylate synthetase-like 2 is a protein that in the house mouse is encoded by the Oasl2 gene. (wikipedia.org)
Figure 2: Postulated G-protein-mediated signal transduction process in normal and regenerating endothelial cells. (nature.com)
It has been recently found that the carboxy-terminal domain (CTD) of rotavirus VP3 has a 2'-5'- phosphodiesterase (PDE) activity that is able to functionally substitute for the PDE activity of the mouse hepatitis virus ns2 protein . (bvsalud.org)
While 2'-5'-oligoadenylate synthetase (OAS)-like gene was upregulated at the 6th hour post infection up to 3.3 fold, Mx protein was downregulated marginally. (usda.gov)
Expression of IFN-inducible protein IP-30 was suppressed up to 2.4 fold but that of interferon-inducible 58 kDa with tetratricopeptide repeats 5 (IFIT-5) was increased up to 20.1 fold in the early stage of infection (6 hrs post infection). (usda.gov)
The PKR is indirectly activated by the dsRNA (actually by 2'-5' oligoadenylate produced by the 2'-5' oligoadenylate-synthetase which is produced due to TLR3 activation), and begins transferring phosphate groups ( phosphorylating ) to a protein known as eIF-2 , a eukaryotic translation initiation factor. (wikidoc.org)
They are important post-transcriptional regulators of gene expression in plants, metazoans, and mammals, and are predicted to control the activity of 30% of all protein-coding genes [ 2 ]. (biomedcentral.com)
Monoclonal Antibodies to Coronavirus SARS-CoV-2 RBD Domain Description :2019 Coronavirus SARS-CoV-2 Spike S1 RBD Protein, human IgG1 Fc tag. (lifetein.com)
Finally, US11 has been recently shown to also counteract the activity of the 2'-5' oligoadenylate synthetase (OAS), a cellular protein critical for host cell defense [ 15 ]. (biomedcentral.com)
In response, cells produce the interferon inducible RNA-dependent protein kinase (PKR) that, when bound to viral dsRNA, phosphorylates eukaryotic initiation factor 2αand attenuates viral protein translation. (uleth.ca)
PRRSV employs −1 PRF directed by RNA secondary and tertiary structures within its viral genome (canonical PRF), as well as a noncanonical −1 and −2 PRF that are stimulated by the interactions of PRRSV nonstructural protein 1β (nsp1β) and host protein poly(C)-binding protein (PCBP) 1 or 2 with the viral genome. (uleth.ca)
The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates. (thermofisher.com)
The expression of IFN-β-responsive genes, including protein kinase R (PKR) and 2′, 5′-oligoadenylate synthetase (OAS), were significantly elevated in the Ad:IFN-β-transduced cells by 12-fold and 25-fold, respectively. (monash.edu)
Interferon stimulates the expression of a number of genes encoding enzymes with antiviral activities, including myxovirus resistance-1 (MxA), 2-5-oligoadenylate synthetase 1 (OAS-1) and double-stranded RNA-dependent protein kinase (PKR). (ox.ac.uk)
Activation of PERK elicits a transient global protein synthesis shutdown through the phosphorylation of the alpha subunit of the eukaryotic translation initiation factor 2 (eIF2α). (ucsb.edu)
OAS31
Conversely, whereas both OAS3_S381 and R381 variants were able to block DENV-1 infection, the anti-DENV-2 activity observed for OAS3_S381 was largely lost for the R381 variant. (nih.gov)
Antiviral2
While both OAS1-p42 and p46 showed antiviral activity against DENV-2, only OAS1-p42 presented anti-DENV-1 activity. (nih.gov)
Oligoadenylate synthetases are antiviral enzymes that counteract viral attack by degrading viral RNA. (embl.de)
Proteins2
Later on, once viral proteins are synthesized, the phosphodiesterase activity of VP3 degrades the cellular 2'-5'-oligoadenylates, which are potent activators of RNase L, preventing its activation. (bvsalud.org)
This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. (thermofisher.com)
Inducing1
Inducing interferon: Abidor activates 2,5-oligoadenylate synthetase (OAS) to induce interferon production, degrade viral mRNA, and prevent viral replication. (pharmaceutical-sale.net)
Synthase1
Here, we identify that host factors allow increased virus replication and cytokine production, providing a mechanism for understanding the severity of virus disease in CF. Increased virus is due to lack of nitric oxide synthase 2 (NOS2) and 2', 5' oligoadenylate synthetase (OAS) 1 induction in response to virus or IFNgamma. (nih.gov)
Molecules1
Instead RNaseL is activated by 2',5'-linked oligoadenylates (2-5As), small RNA-like molecules generated by 2',5'-oligoadenylate synthetases (OASs), which work as direct sensors of double-stranded RNA. (ucsb.edu)
Activation3
Rotavirus Controls Activation of the 2'-5'-Oligoadenylate Synthetase/RNase L Pathway Using at Least Two Distinct Mechanisms. (bvsalud.org)
PERK activation results in expression of CHOP, which turns on the gene encoding the death receptor 5 (DR5), whose ligand-independent activation pushes the cell towards death. (ucsb.edu)
Upon the occupancy of ACE2 by SARS-CoV-2, the increased serum level of free Angiotensin II (Ang II) due to a reduction of ACE2-mediated degradation promotes activation of the NF-kappa B pathway via Ang II type 1 receptor (AT1R), followed by interleukin-6 (IL-6) production. (kegg.jp)
Gene family1
We compared the effect of OAS gene family variants on 2 DENV serotypes in cell culture. (nih.gov)
Enzymes1
Then, the recombinant plasmid was verified by colony PCR (Figure 1 , lane 2) and restriction enzymes digestion (Figure 1 , lane 3). (biomedcentral.com)
Immune3
Among the findings of the transcriptomic analysis was the discovery that the innate immune system's rapid response (six hours after the injections) involved the modulation of four different pathways: TLR (toll-like receptor), RIG-I (viral invasion sensors), OAS (2',5'- oligoadenylate synthetase and RNase L) and oncostatin-M (corresponding to the interleukin-6 family inflammation pathway). (eurekalert.org)
2'-5'-Oligoadenylate synthetase single-nucleotide polymorphisms and haplotypes are associated with variations in immune responses to rubella vaccine. (cdc.gov)
Together, nsp1β and one of the PCBPs act as transactivators that bind a C-rich motif near the shift site to stimulate −1 and −2 PRF, thereby enabling the ribosome to generate two frameshift products that are implicated in viral immune evasion. (uleth.ca)
Differentiation1
Researchers identified that individuals with a deficiency of myeloid differentiation primary response 88 (MyD88) and interleukin-1 receptor-associated kinase 4 (IRAK-4) due to an autosomal recessive condition were highly susceptible to developing hypoxemic coronavirus disease 2019 (COVID-19) pneumonia when infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (news-medical.net)
MeSH1
2',5'-Oligoadenylate Synthetase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
Mice1
therefore we strongly recommend targeting verification of the derived mice with 5' or 3' LRPCR and/or Loss of Allele analysis. (mmrrc.org)
This particular phosphodiesterase cleaves the 2'-5'-phosphodiester bond of the oligoadenylates, antagonizing the OAS / RNase L pathway. (bvsalud.org)
Largely1
This is the largely alpha-helical, C-terminal half of 2'-5'-oligoadenylate synthetase 1, being described as domain 2 of the enzyme and homologous to a tandem ubiquitin repeat. (embl.de)
Fold1
However, cytokines IL-2, IL-6, IL-16, IL-17 as well as IL-4 receptor(IL-4R) and IL-21R were downregulated between 1.2 and 10.8 fold. (usda.gov)
MMRRC Testing of KOMP ES Cell clones includes development of genotyping protocols, genetic verification at the 3' and 5' ends, vector copy number = 1, presence or loss of Y chromosome, and mycoplasma and MPV testing. (mmrrc.org)
The transduction of the adenoviral vector efficiency, ranging from 2% to 100%, was dependent on the multiplicity of infection (moi) (0.4-50 plaque-forming units [pfu]/cell). (monash.edu)
SARS-CoV-2 infects alveolar epithelial cells [mainly alveolar epithelial type 2 (AEC2) cells] through the angiotensin-converting enzyme 2 (ACE2) receptor. (kegg.jp)
Cancer1
While there is some controversy surrounding recent research that claims that miR-155 may display anti-oncogenic or pro-immunological benefits (Cell Rep 2:1697-1709, 2012), most research seems to point to the importance of anti-miRs, with anti-miR-155 in particular, for cancer therapy. (biomedcentral.com)
Expression1
Ex vivo infection of PBLs with VSV elicited upregulated expression of RIG-I, MDA-5, tetherin, IFITM3, and MxA . (karger.com)
Enzyme1
An enzyme that catalyzes the conversion of ATP into a series of (2'-5') linked oligoadenylates and pyrophosphate in the presence of double-stranded RNA. (umassmed.edu)
One of these pathways is the 2'-5'-oligoadenylate synthetase ( OAS )/ RNase L pathway. (bvsalud.org)
Regions1
Secondary structure of the 5′ nontranslated regions of hepatitis C virus and pestivirus genomic RNAs. (microbiologyresearch.org)
Data1
In general, the array data that we enter in GeneNetwork have been log transformed and then z-score normalized, but instead of leaving the mean at 0 and the standard deviation of 1 unit, the data is rescaled to a mean of 8 units with a standard deviation of 2 units (what we call 2Z + 8 normalized data). (genenetwork.org)
Coronavirus disease of 2019 (COVID-19) is a highly contagious respiratory infection that is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (kegg.jp)
2 Department of General Medicine, School of Medicine, College of Medicine, Taipei Medical University Department of Primary Care Medicine, Taipei Medical University Hospital, Taipei City. (nih.gov)