Identification and characterization of riproximin, a new type II ribosome-inactivating protein with antineoplastic activity from Ximenia americana. (1/4)
The aim of this study was to identify and characterize the active component(s) of Ximenia americana plant material used to treat cancer in African traditional medicine. By a combination of preextraction, extraction, ion exchange and affinity chromatography, a mixture of two cytotoxic proteins was isolated. Using degenerated primers designed on the de novo sequence of two tryptic peptides from one of these proteins, a DNA fragment was amplified and the sequence obtained was used to determine the complete cDNA sequence by the RACE method. Sequence analysis and molecular modeling showed that the new protein, riproximin, belongs to the family of type II ribosome inactivating proteins. These results are in good agreement with the ability of riproximin to inhibit protein synthesis in a cell-free system, as well as with the cytotoxicity of riproximin, as demonstrated by its IC50 value of 0.5 pM in MCF7, 1.1 pM in HELA and 0.6 pM in CC531-lacZ cells. To assess the antineoplastic efficacy of the purified riproximin in vivo, the CC531-lacZ colorectal cancer rat metastasis model was used. Significant anticancer activity was found after administration of total dosages of 100 (perorally) and 10 (intraperitoneally) pmol riproximin/kg. These results suggest that riproximin has distinct potential for cancer treatment. (+info)Evaluation of riproximin binding properties reveals a novel mechanism for cellular targeting. (2/4)
(+info)First record of Calliephialtes sittenfeldae associated with the tephritid fruit fly Anastrepha spatulata in Mexico. (3/4)
(+info)Schoepfiajasmins A-H: C-glycosyl dihydrochalcones, dihydrochalcone glycoside, C-glucosyl flavanones, flavanone glycoside and flavone glycoside from the branches of Schoepfia jasminodora. (4/4)
From the branches of Schoepfia jasminodora collected in Okinawa, three new dihydrochalcone C-glycosides, one dihydrochalcone di-O-glucopyranoside, two flavanone C-glycosides, one flavanone O-glycoside and one flavone O-glycoside were isolated. Their structures were elucidated by extensive study of one- and two-dimensional NMR spectroscopic data. (+info)Olacaceae is a family of flowering plants characterized by simple, alternate leaves, small flowers with 4-5 sepals and petals, and drupaceous fruits, including several genera such as Olax, Ximenia, and Strombosia, found primarily in tropical regions worldwide.
I apologize for any confusion, but "Olacaceae" is not a medical term. It is a taxonomic category in botany, specifically the name of a family of flowering plants that includes around 28 genera and 600 species. These plants are found primarily in tropical regions and have simple, actinomorphic flowers and fleshy fruits. Examples of plants in this family include Olax, Ximenia, and Heisteria.
It is not uncommon for there to be some overlap between medical and botanical terminology, as many medicinal treatments are derived from plants. However, in this case, "Olacaceae" does not have a specific medical definition.