Phenylalanine Ammonia-Lyase
Apiaceae
Ammonia-Lyases
Plants
Rapid increase of NO release in plant cell cultures induced by cytokinin. (1/38)
4,5-Diaminofluorescein, a fluorescence indicator for NO, was applied to detect the release of NO from plant cells. NO production was increased within 3 min when plant cell cultures (Arabidopsis, parsley, and tobacco) were treated by cytokinin and was dose-dependent and signal-specific in that other plant hormones and inactive cytokinin analog were not effective in stimulating of NO release. The response was quenched by addition of 2-(aminoethyl)-2-thiopseudourea, an inhibitor of the animal NO synthase, and by addition of an NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-1-oxy-3-oxide. These results imply that NO may act in cytokinin signal transduction. (+info)Crosstalk among stress responses in plants: pathogen defense overrides UV protection through an inversely regulated ACE/ACE type of light-responsive gene promoter unit. (2/38)
Plants often have to cope with two or more environmental hazards simultaneously. Such coincidences require instantaneous decisions on relative severity and consequential crosstalk between the respective signaling cascades. Among the frequently encountered threats are pathogen infections and UV irradiation, both of which trigger specifically targeted defense responses by means of changes in gene transcription rates. In Petroselinum crispum, pathogen defense has been shown to be associated with extensive metabolic reprogramming, including strong repression of the UV-protective flavonoid biosynthetic pathway. Here we show that one of the involved genes, encoding acyl-CoA oxidase, responds positively to UV light and negatively to a pathogen-derived elicitor through an inversely regulated promoter unit consisting of two almost identical ACGT-containing elements (ACEs). This unit, when either introduced into an unrelated promoter or generated by mutation of a differently composed unit, confers the same type of response pattern on the recipient genes, confirming its general functionality at a convergence site of two largely distinct signaling pathways. Similarly large, rapid, and partly inverse effects of UV light and elicitor were observed for several mRNAs encoding common plant regulatory factors (CPRFs) that exhibit distinct dimerization and DNA-binding properties. This striking coincidence suggests a major role of common plant regulatory factors in mediating the apparent switch in the function of ACGT-containing elements from positive UV light to negative elicitor or pathogen responsiveness. (+info)Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley. (3/38)
Two new WRKY transcription factors from parsley (Petroselinum crispum), WRKY4 and WRKY5, were isolated using the yeast one-hybrid system. In yeast, both proteins interacted sequence-specifically with W boxes (TTGACC) and activated transcription. They appear to contain functional leucine zippers, which increase their affinities for W boxes. Co-transfection experiments in parsley protoplasts confirmed their in vivo-binding specificity for W boxes. Elicitor-mediated expression of the WRKY5 gene, the first parsley member of the group III family of WRKY proteins, is extremely transient, with high mRNA levels occurring within a time window of less than 1 h. WRKY4 and -5, as well as the previously identified parsley transcription factors WRKY1 and -3, are encoded by immediate early elicitor-activated genes that differ in their sensitivity to cycloheximide (CHX) and their activation kinetics. We propose that a number of the pathways activated during the plant defense response require the induction of several distinct WRKY transcription factors with different DNA binding-site preferences to fine-tune the activation of a wide spectrum of target genes. (+info)Pretreatment with salicylic acid primes parsley cells for enhanced ion transport following elicitation. (4/38)
Pretreatment with salicylic acid (SA), an inducer of plant disease resistance, enhanced the capacity of parsley cells for the induction of a rapid K(+)/pH response and the subsequent coumarin (phytoalexin) secretion. In SA-primed cells, a low elicitor dose induced these two responses to a similar extent as did a high elicitor dose in non-primed cells. These observations suggest that the SA-mediated augmentation of the early K(+)/pH response may contribute to the enhancement of subsequent coumarin secretion. As the amphotericin B-induced K(+)/pH response was not enhanced in SA-primed cells, it is concluded that signaling components that are improved by priming are located between elicitor signal perception and the plasma membrane transporters mediating the K(+)/pH response. (+info)An active site homology model of phenylalanine ammonia-lyase from Petroselinum crispum. (5/38)
The plant enzyme phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) shows homology to histidine ammonia-lyase (HAL) whose structure has been solved by X-ray crystallography. Based on amino-acid sequence alignment of the two enzymes, mutagenesis was performed on amino-acid residues that were identical or similar to the active site residues in HAL to gain insight into the importance of this residues in PAL for substrate binding or catalysis. We mutated the following amino-acid residues: S203, R354, Y110, Y351, N260, Q348, F400, Q488 and L138. Determination of the kinetic constants of the overexpressed and purified enzymes revealed that mutagenesis led in each case to diminished activity. Mutants S203A, R354A and Y351F showed a decrease in kcat by factors of 435, 130 and 235, respectively. Mutants F400A, Q488A and L138H showed a 345-, 615- and 14-fold lower kcat, respectively. The greatest loss of activity occurred in the PAL mutants N260A, Q348A and Y110F, which were 2700, 2370 and 75 000 times less active than wild-type PAL. To elucidate the possible function of the mutated amino-acid residues in PAL we built a homology model of PAL based on structural data of HAL and mutagenesis experiments with PAL. The homology model of PAL showed that the active site of PAL resembles the active site of HAL. This allowed us to propose possible roles for the corresponding residues in PAL catalysis. (+info)Mitogen-activated protein kinases play an essential role in oxidative burst-independent expression of pathogenesis-related genes in parsley. (6/38)
Plants are continuously exposed to attack by potential phytopathogens. Disease prevention requires pathogen recognition and the induction of a multifaceted defense response. We are studying the non-host disease resistance response of parsley to the oomycete, Phytophthora sojae using a cell culture-based system. Receptor-mediated recognition of P. sojae may be achieved through a thirteen amino acid peptide sequence (Pep-13) present within an abundant cell wall transglutaminase. Following recognition of this elicitor molecule, parsley cells mount a defense response, which includes the generation of reactive oxygen species (ROS) and transcriptional activation of genes encoding pathogenesis-related (PR) proteins or enzymes involved in the synthesis of antimicrobial phytoalexins. Treatment of parsley cells with the NADPH oxidase inhibitor, diphenylene iodonium (DPI), blocked both Pep-13-induced phytoalexin production and the accumulation of transcripts encoding enzymes involved in their synthesis. In contrast, DPI treatment had no effect upon Pep-13-induced PR gene expression, suggesting the existence of an oxidative burst-independent mechanism for the transcriptional activation of PR genes. The use of specific antibodies enabled the identification of three parsley mitogen-activated protein kinases (MAPKs) that are activated within the signal transduction pathway(s) triggered following recognition of Pep-13. Other environmental challenges failed to activate these kinases in parsley cells, suggesting that their activation plays a key role in defense signal transduction. Moreover, by making use of a protoplast co-transfection system overexpressing wild-type and loss-of-function MAPK mutants, we show an essential role for post-translational phosphorylation and activation of MAPKs for oxidative burst-independent PR promoter activation. (+info)Non-self recognition, transcriptional reprogramming, and secondary metabolite accumulation during plant/pathogen interactions. (7/38)
Disease resistance of plants involves two distinct forms of chemical communication with the pathogen: recognition and defense. Both are essential components of a highly complex, multifaceted defense response, which begins with non-self recognition through the perception of pathogen-derived signal molecules and results in the production, inter alia, of antibiotically active compounds (phytoalexins) and cell wall-reinforcing material around the infection site. To elucidate the molecular details and the genomic basis of the underlying chains of events, we used two different experimental systems: suspension-cultured cells of Petroselinum crispum (parsley) and wild-type as well as mutant plants of Arabidopsis thaliana. Particular emphasis was placed on the structural and functional identification of signal and defense molecules, and on the mechanisms of signal perception, intracellular signal transduction and transcriptional reprogramming, including the structural and functional characterization of the responsible cis-acting gene promoter elements and transacting regulatory proteins. Comparing P. crispum and A. thaliana allows us to distinguish species-specific defense mechanisms from more universal responses, and furthermore provides general insights into the nature of the interactions. Despite the complexity of the pathogen defense response, it is experimentally tractable, and knowledge gained so far has opened up a new realm of gene technology-assisted strategies for resistance breeding of crop plants. (+info)Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley. (8/38)
Flavone synthases (FNSs) catalyze the oxidation of flavanones to flavones, i.e. the formation of apigenin from (2S)-naringenin. While many plants express a microsomal-type FNS II, the soluble FNS I appears to be confined to a few species of the Apiaceae and was cloned recently from parsley plants. FNS I belongs to the Fe(II)/2-oxoglutarate-dependent dioxygenases characterized by short conserved sequence elements for cofactor binding, and its evolutionary context and mode of action are under investigation. Using a homology-based reverse transcription polymerase chain reaction approach, two additional flavonoid-specific dioxygenases were cloned from immature parsley leaflets, which were identified as flavanone 3beta-hydroxylase (FHT) and flavonol synthase (FLS) after expression in yeast cells. Sequence alignments revealed marginal differences among the parsley FNS I and FHT polypeptides of only 6%, while much less identity (about 29%) was observed with the parsley FLS. Analogous to FNS I, FLS oxidizes the flavonoid gamma-pyrone by introducing a C2, C3 double bond, and (2R,3S)-dihydrokaempferol (cis-dihydrokaempferol) was proposed recently as the most likely intermediate in both FNS I and FLS catalysis. Incubation of either FNS I or FLS with cis-dihydrokaempferol exclusively produced kaempferol and confirmed the assumption that flavonol formation occurs via hydroxylation at C3 followed by dehydratation. However, the lack of apigenin in these incubations ruled out cis-dihydrokaempferol as a free intermediate in FNS I catalysis. Furthermore, neither (+)-trans-dihydrokaempferol nor unnatural (-)-trans-dihydrokaempferol and 2-hydroxynaringenin served as a substrate for FNS I. Overall, the data suggest that FNS I has evolved uniquely in some Apiaceae as a paraphyletic gene from FHT, irrespective of the fact that FNS I and FLS catalyze equivalent desaturation reactions. (+info)"Petroselinum" is the genus name for a group of plants that include several types of parsley. The most common variety is often used as a herb in cooking and is known as "Petroselinum crispum." It is native to the Mediterranean region and is now grown worldwide. Parsley has a bright, fresh flavor and is often used as a garnish or added to recipes for additional flavor. In addition to its use as a culinary herb, parsley has also been used in traditional medicine for its potential diuretic and digestive properties. However, it's important to note that the scientific evidence supporting these uses is limited, and more research is needed before any firm conclusions can be drawn.
Phenylalanine Ammonia-Lyase (PAL) is a enzyme that catalyzes the non-oxidative deamination of phenylalanine to trans-cinamic acid, releasing ammonia in the process. This reaction is a key step in the biosynthesis of various aromatic compounds in plants and microorganisms. In humans, PAL is not normally present, but its introduction through gene therapy has been studied as a potential treatment for phenylketonuria (PKU), a genetic disorder characterized by an inability to metabolize phenylalanine properly, leading to its accumulation in the body and potential neurological damage.
Apiaceae is a family of flowering plants also known as Umbelliferae. It includes aromatic herbs and vegetables such as carrots, parsley, celery, fennel, and dill. The plants in this family are characterized by their umbrella-shaped clusters of flowers (umbels) and hollow stems. Some members of Apiaceae contain toxic compounds, so caution should be taken when identifying and consuming wild plants from this family.
Ammonia-lyases are a class of enzymes that catalyze the removal of an amino group from a substrate, releasing ammonia in the process. These enzymes play important roles in various biological pathways, including the biosynthesis and degradation of various metabolites such as amino acids, carbohydrates, and aromatic compounds.
The reaction catalyzed by ammonia-lyases typically involves the conversion of an alkyl or aryl group to a carbon-carbon double bond through the elimination of an amine group. This reaction is often reversible, allowing the enzyme to also catalyze the addition of an amino group to a double bond.
Ammonia-lyases are classified based on the type of substrate they act upon and the mechanism of the reaction they catalyze. Some examples of ammonia-lyases include aspartate ammonia-lyase, which catalyzes the conversion of aspartate to fumarate, and tyrosine ammonia-lyase, which converts tyrosine to p-coumaric acid.
These enzymes are important in both plant and animal metabolism and have potential applications in biotechnology and industrial processes.
I apologize for any confusion, but "plants" is not a term that has a medical definition. The term "plants" refers to a large and diverse group of organisms that obtain their energy through photosynthesis, which is the process of converting sunlight into chemical energy. Plants are typically characterized by having cells with cell walls containing cellulose, chloroplasts containing the pigment chlorophyll, and the ability to synthesize their own food through photosynthesis.
In a medical or biological context, you might be thinking of "plant-based" or "phytomedicine," which refer to the use of plants or plant extracts as a form of medicine or treatment. Phytomedicines have been used for thousands of years in many traditional systems of medicine, and some plant-derived compounds have been found to have therapeutic benefits in modern medicine as well. However, "plants" itself does not have a medical definition.
Acyltransferases are a group of enzymes that catalyze the transfer of an acyl group (a functional group consisting of a carbon atom double-bonded to an oxygen atom and single-bonded to a hydrogen atom) from one molecule to another. This transfer involves the formation of an ester bond between the acyl group donor and the acyl group acceptor.
Acyltransferases play important roles in various biological processes, including the biosynthesis of lipids, fatty acids, and other metabolites. They are also involved in the detoxification of xenobiotics (foreign substances) by catalyzing the addition of an acyl group to these compounds, making them more water-soluble and easier to excrete from the body.
Examples of acyltransferases include serine palmitoyltransferase, which is involved in the biosynthesis of sphingolipids, and cholesteryl ester transfer protein (CETP), which facilitates the transfer of cholesteryl esters between lipoproteins.
Acyltransferases are classified based on the type of acyl group they transfer and the nature of the acyl group donor and acceptor molecules. They can be further categorized into subclasses based on their sequence similarities, three-dimensional structures, and evolutionary relationships.
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Petroselinum - Wikipedia
Petroselinum
Carum Petroselinum Seed Oil (Petersilienöl) | LUSH
Petroselinum crispum tuberosum Hamburg Parsley PFAF Plant Database
Petroselinum crispum - definition and meaning
Antiosteoporotic effect of Petroselinum crispum, Ocimum basilicum and
Petroselinum hortense in Flora of Pakistan @ efloras.org
Petroselinum crispum - Parsley
What does Petroselinum crispum mean?
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The Effects of Petroselinum Crispum on Estrogen Receptor-positive Benign and Malignant Mammary Cells (MCF12A/MCF7) | Anticancer...
In vitro anti-nephrotoxic potential of Ammi visnaga, Petroselinum crispum, Hordeum vulgare, and Cymbopogon schoenanthus seed or...
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Petroselinum crispum var. neapolitanum | Flat Leaf Italian Parsley | 1 - buyrareseeds
Parsley16
- Petroselinum is a genus of two parsley species of flowering plants in the family Apiaceae, native to western and southern Europe and northern Africa. (wikipedia.org)
- The species of this genus are: Petroselinum crispum (garden parsley) from southern Europe and northern Africa (southern Italy, Greece, Algeria, Tunisia). (wikipedia.org)
- Petroselinum segetum (corn parsley) from western Europe (Great Britain and the Netherlands south through France to Italy, Spain and Portugal). (wikipedia.org)
- species within the genus Petroselinum - curly-leaved parsley. (wordnik.com)
- Petroselinum crispum, commonly known as parsley, is a species of flowering plant in the family Apiaceae, native to the central and eastern Mediterranean region. (definitions.net)
- Petroselinum crispum Triple Curled Parsley is a highly nutritious herb commonly used in Middle Eastern, European and American cooking. (sheffields.com)
- Enjoy the nutritious and flavorful benefits of Petroselinum crispum Triple Curled Parsley today! (sheffields.com)
- Petroselinum Crispum Oil: The name comes from the Greek word petros, which means stone, as parsley naturally grows in stony terrain. (razskincare.com)
- Napolitian Parsley Plant (Petroselinum crispum var. (spicegarden.eu)
- Hello, is this parsley (Petroselinum crispum) used as Carum petroselinum, Apium petroselinum, Petroselinum lativum (A Modern Herbal listings) or Petroselinum sativum? (strictlymedicinalseeds.com)
- Oil of parsley fruits (Petroselinum sativum Hoffm. (iso.org)
- Parsley ( Petroselinum crispum ) is a plant widely known for its properties as an aromatic herb. (botanical-online.com)
- The parsley 'Amsterdamse Snij' (Petroselinum crispum) has many culinary uses. (bakker.com)
- Parsley ( Petroselinum crispum and P. sativum ) is a member of the Apiaceae family of plants. (encyclopedia.com)
- Parsley herb flower head, parsley flowers (Petroselinum crispum neapolitanum), New Zealand (NZ). (naturespic.co.nz)
- Parsley herb flower head, parsley flowers (Petroselinum crispum neapolitanum), New Zealand (NZ), stock photo. (naturespic.co.nz)
Sativum2
- Petroselinum sativum Hoffm. (theferns.info)
- Petroselinum hortense, Petroselinum sativum, Apium petroselinum ) in whole, cut leaves or rubbed (ground) form. (iso.org)
Apium1
- Apium petroselinum L. (theferns.info)
Hortense1
- Petroselinum hortense Hoffm. (efloras.org)
BIENNIAL2
- Petroselinum crispum tuberosum is a BIENNIAL growing to 0.6 m (2ft) by 0.3 m (1ft in). (pfaf.org)
- countable , uncountable ) A bright green, biennial herb , Petroselinum crispum , having many cultivars . (wiktionary.org)
Crispum tuberosum1
- Petroselinum crispum tuberosum - (Bernh. (pfaf.org)
Genus1
- ISBN 0-340-40170-2 Interactive Flora of NW Europe: Petroselinum species list[permanent dead link] and genus description[permanent dead link] Lewis, Charlton T. (wikipedia.org)
Mill1
- Petroselinum crispum (Mill. (henriettes-herb.com)
Roots1
- Are the roots of Petroselinum crispum usable as well? (strictlymedicinalseeds.com)
Translations1
- This dictionary definitions page includes all the possible meanings, example usage and translations of the word Petroselinum crispum . (definitions.net)
Page1
- Help support Wordnik (and make this page ad-free) by adopting the word Petroselinum crispum . (wordnik.com)
Europe1
- Native to Southern Europe, Petroselinum crispum var. (gardenia.net)
Media1
- Wikimedia Commons has media related to Petroselinum. (wikipedia.org)
Crispum15
- The species of this genus are: Petroselinum crispum (garden parsley) from southern Europe and northern Africa (southern Italy, Greece, Algeria, Tunisia). (wikipedia.org)
- Your search for PETROSELINUM CRISPUM did not return any results. (nih.gov)
- Parsley leaf (petroselinum crispum) is a versatile herb that can be used both fresh and dried to add flavor to dishes. (glenbrookfarm.com)
- Browse pictures and read growth / cultivation information about Petroselinum, Flat-Leaf Parsley, Italian Parsley (Petroselinum crispum var. (casacerrado.org.br)
- Native to Europe and the Mediterranean, Petroselinum crispum (Parsley) is a bushy biennial forming a dense rosette of rich green, triangular, aromatic leaves finely divided into curly or flat leaflets. (casacerrado.org.br)
- Also suited as a … Plain Flat Leaf Italian Parsley Culinary - Petroselinum Crispum - 150 Seeds quantity. (casacerrado.org.br)
- Scientific Name: Petroselinum crispum Plant Type: Biennial herb Light: Partial shade to full sun Water: When it comes to watering the parsley plant, keep its potting soil moist, and do not let it dry out. (casacerrado.org.br)
- Find help & information on Petroselinum crispum 'Italian' Italian parsley from the RHS Multi-use crop for raising for pot herb sales, or for full grown plants. (casacerrado.org.br)
- Latin Name: Petroselinum crispum Family: Apiaceae The listings of research below represent a compilation of scientific articles found on the species, with a very brief overview description of each article/study. (earthtokathy.com)
- Petroselinum crispum (Mill. (ngpherbaria.org)
- Latin name: Petroselinum crispum var. (terrepromise.ca)
- Conclusion: These results suggested that ethanolic extract of Petroselinum crispum reduced the dysfunction in rats kidney caused by prostadin-induced abortion and could have beneficial effect in reducing the progression of prostaglandin-induced edema. (virascience.com)
- Petroselinum crispum is a multipurpose herb in traditional medicine which contains essential amino acids, vitamins and numerous nutrients. (virascience.com)
- This study aimed to determine the effects of Petroselinum crispum extract on milk production parameters in female rats. (virascience.com)
- One of the best herbs for container growing is parsley (Petroselinum crispum). (ehow.com)
SATIVUM5
- Petroselinum hortense, Petroselinum sativum, Apium petroselinum ) in whole, cut leaves or rubbed (ground) form. (iso.org)
- The efficacy of household decontamination methods at reducing Listeria monocytogenes on fresh lettuce (Lactuca sativa), cucumber (Cucumis sativus) and parsley (Petroselinum sativum) was studied. (nih.gov)
- Parsley ( Petroselinum sativum ) is a member of the edible Umbelliferae plants. (nih.gov)
- 1. Petroselinum sativum protects HepG2 cells from cytotoxicity and oxidative stress induced by hydrogen peroxide. (nih.gov)
- 3. Cytotoxicity assessments of Portulaca oleracea and Petroselinum sativum seed extracts on human hepatocellular carcinoma cells (HepG2). (nih.gov)
Parsley Petroselinum1
- Italian Parsley Petroselinum neopolitanum. (casacerrado.org.br)
Carum2
- Carum petroselinum (L.) Benth. (ngpherbaria.org)
- Parsley ( Carum petroselinum ) leaf, seed, and root contain the volatile oils apiol and myristicin, which is pharmacologically active, as well as flavonoids, beta-phellandrene, bergapten, and vitamins A and C. Warm compresses or poultices of parsley have been used to treat breast engorgement and mastalgia. (nih.gov)
Biennial1
- Petroselinum segetum is a BIENNIAL growing to 1 m (3ft 3in). (pfaf.org)
Segetum2
- Petroselinum segetum (corn parsley) from western Europe (Great Britain and the Netherlands south through France to Italy, Spain and Portugal). (wikipedia.org)
- Petroselinum segetum - (L.)Koch. (pfaf.org)
Species2
- Petroselinum is a genus of two parsley species of flowering plants in the family Apiaceae, native to western and southern Europe and northern Africa. (wikipedia.org)
- ISBN 0-340-40170-2 Interactive Flora of NW Europe: Petroselinum species list[permanent dead link] and genus description[permanent dead link] Lewis, Charlton T. (wikipedia.org)