• TEC development require activity of other molecules and transcriptional regulators, such as protein 63 (p63) that is involved in homeostasis of various epithelial lineages, chromobox homolog 4 (Cbx4) which regulates cell proliferation and differentiation, fibroblast growth factors Fgf7 and Fgf10 that initiate TEC expansion, TNFT, CD40, lymfotoxin β receptor (LTβR) and Hedgehog signaling pathway, which could reduce TEC cells in fetal and postnatal thymus. (wikipedia.org)
  • Cytokines, growth factors, Wnt ligands, SPMs, and MMPs released in the wound microenvironment in response to injury support epithelial cell proliferation as well as migration. (jci.org)
  • The hedgehog pathway regulates epithelial-mesenchymal interactions, differentiation, proliferation and survival during development. (rti.org)
  • These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and increase apoptosis in both normal and in tumoral breast cells, being the effect more pronounced in the tumoral cell line. (scirp.org)
  • Stored and non-stored cultures were analyzed for cell viability, amniotic membrane and epithelial sheet thickness, and a panel of immunohistochemical markers for immature cells (ΔNp63α, p63, Bmi-1, C/EBP∂, ABCG2 and K19), differentiated cells (K3 and Cx43), proliferation (PCNA), and apoptosis (Caspase-3). (datadryad.org)
  • The present study investigated the effects of GSK2606414 on proliferation, apoptosis, and the expression of activating transcription factor 4 (ATF4), CCAAT/enhancer‑binding protein homologous protein (CHOP) and vascular endothelial growth factor (VEGF) in human retinal pigment epithelial (RPE) cells under endoplasmic reticulum (ER) stress. (spandidos-publications.com)
  • Cell proliferation was assessed using the Cell Counting kit‑8 cell viability assay. (spandidos-publications.com)
  • GSK2606414 treatment inhibited RPE cell proliferation in a dose‑dependent manner, however it did not induce apoptosis. (spandidos-publications.com)
  • Given the strong link between the regulation of some of these genes and the growth and dissemination of gastrointestinal cancers, we believe their aberrant expression in UC may provide a mechanism for epithelial hyper-proliferation and, in the context of malignant transformation, for tumour growth. (bmj.com)
  • Among the genes that remain deregulated during remission, we identify several that are expressed by epithelial cells and are involved in epithelial cell proliferation, resistance to apoptosis and response to stress. (bmj.com)
  • Here, we have investigated the role of ChoK in the development of breast cancer and found that ChoK is both necessary and sufficient for growth factor-induced proliferation in primary human mammary epithelial cells and an absolute requirement for the specific mitogenic response to heregulin in breast tumor-derived cells. (aacrjournals.org)
  • These results demonstrate that ChoK plays an essential role in both normal human mammary epithelial cell proliferation and breast tumor progression. (aacrjournals.org)
  • The EasySep™ Mouse Epithelial Cell Enrichment Kit II is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by immunomagnetic negative selection. (stemcell.com)
  • Proteomics of the secretomes of bovine mammary epithelial. (au.dk)
  • High-efficiency neoplastic transformation of mouse mammary epithelial cells in primary collagen gel culture was induced by N-methyl-N-nitrosourea (MNU). (eurekamag.com)
  • Mammary epithelial cells, isolated from virgin BALB/c mice, were embedded within collagen gels and grown in a serum-free medium containing prolactin, progesterone, and linoleic acid. (eurekamag.com)
  • Eleven to 14 days after the final carcinogen treatment, the cells were removed from the collagen gels and injected into the cleared mammary fat pads of syngeneic hosts to assay for transformed cell populations. (eurekamag.com)
  • Control cells cultured for the same periods of time as MNU-treated cells produced only ductal outgrowths that were morphologically similar to those found in the mammary glands of adult virgin hosts. (eurekamag.com)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • While considerable research has been performed On the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells. (harvard.edu)
  • As an HuR/mRNA disruptor, KH-3 treatment successfully mimicked the effects of HuR knockdown in pancreatic cancer cell lines: KH-3 significantly inhibited pancreatic cancer cell migration, invasion, tumor sphere formation and EMT. (ku.edu)
  • Here we show that expression of the EMT master inducer Snail in primary adult Drosophila intestinal tumors leads to the dissemination of tumor cells and formation of macrometastases. (whiterose.ac.uk)
  • Snail drives an EMT in tumor cells, which, although retaining some epithelial markers, subsequently break through the basal lamina of the midgut, undergo a collective migration and seed polyclonal metastases. (whiterose.ac.uk)
  • Tumor recurrence, acquired resistance and metastasis have severely limited the effect of clinical treatments for epithelial ovarian cancer. (physiciansweekly.com)
  • A platinum(II) complex (HY1-Pt) owning casein kinase 2 specificity reported in our recent research was herein applied to treat cisplatin-sensitive and cisplatin-resistant epithelial ovarian cancers, respectively, anticipating to achieve high anti-tumor efficacy. (physiciansweekly.com)
  • HY1-Pt showed highly efficient anti-tumor effect with low toxicity for either cisplatin-sensitive or cisplatin-resistant epithelial ovarian cancer both in vitro and in vivo. (physiciansweekly.com)
  • Moreover, HY1-Pt could suppress tumor migration and invasion in vitro and in vivo, further proving that HY1-Pt can be a potent novel platinum(II) agent for cisplatin-resistant epithelial ovarian cancer treatment. (physiciansweekly.com)
  • Using 6 biphasic clear cell renal cell carcinoma cases we show that sarcomatoid clear cell renal cell carcinoma shares characteristic markers associated with loss of von Hippel-Lindau tumor suppressor with conventional clear cell renal cell carcinoma and also exhibits a markedly higher proliferative index. (lu.se)
  • Cell viability was estimated by cell counting kit-8 and cell apoptosis by flow cytometry. (karger.com)
  • In LPS-induced ARDS cell model, astragaloside IV up-regulated cell viability, SOD activity and ZO-1 and LC3B I expressions but down-regulated cell apoptosis, TNF-α, IL-6, LC3B II, Beclin-1 and atg5 expressions and LDH and MDA levels. (karger.com)
  • Astragaloside IV might suppress autophagy initiation directly or indirectly through suppressing the oxidative stress and inflammatory response, which further enhances the cell viability and tight junction and reduces apoptosis in LPS-stimulated pulmonary endothelial ARDS cell model, thus exerting its therapeutic function in ARDS. (karger.com)
  • found that FWGE can promote apoptosis in different types of cancer cell lines such as SNU-5, MKN-45, SNU-620, SNU-1, and SNU-16. (hindawi.com)
  • FWGE has significant antiproliferative effects and kills tumour cells by the induction of apoptosis via the caspase-poly (ADP-ribose) polymerase pathway [ 9 ]. (hindawi.com)
  • We also include cell alignment, cell-specific mechanical properties, cell growth, division and apoptosis as well as a flexible, dynamically changing boundary of the epithelial sheet. (cam.ac.uk)
  • Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combina tion or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). (scirp.org)
  • After hCG, Ang-(1 - 7), hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments, MCF7 displayed cell viability decrease and mid-apoptosis increase. (scirp.org)
  • These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. (scirp.org)
  • In summary, cell viability was decreased and apoptosis (initial, mid and late) was in creased after hCG and/or Ang-(1 - 7) peptides treatments. (scirp.org)
  • Disruption of PERK activity has been demonstrated to reduce hydroquinone-induced apoptosis and hypoxia-induced VEGF expression in human RPE cells in vitro ( 5 , 21 ). (spandidos-publications.com)
  • Loss-of-function and gain-of-function approaches were applied in cellular assays, including cell viability, migration, invasion, and apoptosis. (jcancer.org)
  • Twenty-nine of these genes were significantly regulated in UC-in-remission subjects compared with non-IBD controls, including a large number of epithelial cell-expressed genes such as REG4, S100P, SERPINB5, SLC16A1, DEFB1, AQP3 and AQP8, which modulate epithelial cell growth, sensitivity to apoptosis and immune function. (bmj.com)
  • In early experiments, physical separation of mesenchymal and epithelial stroma from embryonic murine thymus resulted in defective thymus development when epithelium was cultured in vitro in the absence of mesenchyme ( 7 ). (frontiersin.org)
  • MLE-12 cells were induced by LPS to construct an ARDS model in vitro. (karger.com)
  • We determined the effect of vapour on oxidative stress-induced, PAFR-dependent pneumococcal adhesion to airway epithelial cells in vitro , and on pneumococcal colonisation in the mouse nasopharynx. (ersjournals.com)
  • Nicotine-containing and nicotine-free E-cigarette vapour increased pneumococcal adhesion to airway cells in vitro . (ersjournals.com)
  • For example, we found that cigarette smoke extract, fossil fuel-derived particulate matter and welding fumes, by means of the induction of oxidative stress, upregulate PAFR-dependent pneumococcal adhesion to lower airway cells in vitro [ 8 - 10 ]. (ersjournals.com)
  • Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
  • To the best of our knowledge, the present study is the first to demonstrate that GSK2606414 has a potential antiproliferative effect in RPE cells in vitro. (spandidos-publications.com)
  • Transforming growth factor β1 exposure of in vitro cultured primary clear cell renal cell carcinoma cells resulted in cells adopting a mesenchymal morphology similar to sarcomatoid clear cell renal cell carcinoma. (lu.se)
  • For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5mM), and/or cysteamine (250muM, an autophagy inducer and anti-oxidant drug), for 6hrs. (who.int)
  • Thymic epithelial cells (TECs) are specialized cells with high degree of anatomic, phenotypic and functional heterogeneity that are located in the outer layer (epithelium) of the thymic stroma. (wikipedia.org)
  • Cortical thymic epithelial cells (cTECs) are presented in the outer thymic cortex region, in comparison with medullary thymic epithelial cells (mTECs) located in the inner thymic medulla. (wikipedia.org)
  • Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)
  • Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
  • The adult alae are commonly used as an indicator of hypodermal seam cell terminal differentiation. (wormatlas.org)
  • The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
  • Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
  • Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
  • In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
  • Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
  • LPS effectively inhibited cell viability and LC3B I expression and enhanced LC3B II, Beclin-1 and atg5 expressions in MLE-12 cells. (karger.com)
  • 3-methyladenine promoted cell viability and ZO-1 expression, down-regulated Beclin-1 and atg5 expression, while Rapamycin (Rap) had an opposite effect. (karger.com)
  • Astragaloside IV suppressed cell viability and ZO-1 expression after the Rap treatment. (karger.com)
  • However, cyclopamine reduced viability of cancer epithelial cell lines, including MDA-MB-435, but did not specifically affect fibroblasts or epithelial cells from benign breast, including MCF10AT. (rti.org)
  • We also observed cell viability decrease in MCF10A after Ang-(1 - 7), Ang-(1 - 7) Fmoc and hCG + AngII Toac treatments. (scirp.org)
  • Aim/Purpose of the Study: To develop a one-week storage method, without serum and xenobiotics, that would maintain cell viability, morphology, and phenotype of cultured human limbal epithelial sheets. (datadryad.org)
  • Results: The cell viability of the cultures was 98 ± 1% and remained high after storage. (datadryad.org)
  • Conclusion: Limbal epithelial cells cultured from explants on amniotic membrane can be stored at 23°C in both serum-free and xenobiotic-free media, with sustained cell viability, ultrastructure, and ΔNp63α-positivity after both 4 and 7 days. (datadryad.org)
  • Data demonstrated that Pao and Rau preferentially inhibited the viability of pancreatic CSC population, compared with the whole cancer cell population. (ku.edu)
  • These cells are also called thymic epithelial progenitors cells (TEPCs) and they provide that cortical and medullary epithelial thymocytes share an origin in the postnatal thymus. (wikipedia.org)
  • In this study, we utilized primary normal human bronchial epithelial (NHBE) cells that are cultured at an air-liquid interface (ALI) to mimic the in vivo airway characteristics 30 . (nature.com)
  • A graphical representation of variants found in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) stock P1 and P2, as well as the apical washes from SARS-CoV-2-infected monkey and cat airway epithelial cell cultures with either P1 or P2 stock 96 hpi at 33°C or 37°C. SARS-CoV-2/Wuhan-Hu-1 (GenBank accession no. (cdc.gov)
  • Since oxidative stress in airway cells increases platelet-activating factor receptor (PAFR) expression, and PAFR is co-opted by pneumococci to adhere to host cells, we hypothesised that E-cigarette vapour increases pneumococcal adhesion to airway cells. (ersjournals.com)
  • To establish firm adhesion, pneumococci co-opt host-expressed platelet-activating factor receptor (PAFR), and then use the receptor as a Trojan horse to enter airway cells as the receptor is internalised [ 7 ]. (ersjournals.com)
  • Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
  • The relative selectivity of COX inhibitors has not been studied in human airway cells. (ersjournals.com)
  • A number of COX inhibitors in cultured human airway cells were compared which exclusively express either COX 1 (primary degree cultured human airway smooth muscle (HASM) cells) or COX 2 (A549 pulmonary epithelial cell-line) as measured by Western blotting. (ersjournals.com)
  • In conclusion the present study has shown that cyclo-oxygenase inhibitors have a range of selectivities for cyclo-oxygenase 1 and cyclo-oxygenase 2 in intact human airway cells. (ersjournals.com)
  • Nasal epithelial PAFR was assessed in non-vaping controls, and in adults before and after 5 min of vaping. (ersjournals.com)
  • There was no difference in baseline nasal epithelial PAFR expression between vapers (n=11) and controls (n=6). (ersjournals.com)
  • Here, we use primary nasal epithelial cells (NECs) from children and adults, differentiated at an air-liquid interface to show that the ancestral SARS-CoV-2 replicates to significantly lower titers in the NECs of children compared to those of adults. (plos.org)
  • Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
  • Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
  • Signaling at short range from urogenital sinus mesenchymal cells to cells of the urogenital epithelium resulting in the epithelial cells adopting a prostatic fate. (mcw.edu)
  • The formation of CSCs is highly associated with cancer cell epithelial-mesenchymal transition (EMT), which contributes to the plasticity of cancer cells and comprises an initial step for metastasis. (ku.edu)
  • Snail-mediated epithelial-mesenchymal transition (EMT) process plays a fundamental role in facilitating pancreatic ductal adenocarcinoma (PDAC) stemness and metastasis. (jcancer.org)
  • Overexpression of miR-30b led to reduced expression of mesenchymal marker N-cadherin and the upregulation of epithelial marker E-cadherin. (jcancer.org)
  • Collective cell migration and metastases induced by an epithelial-to-mesenchymal transition in Drosophila intestinal tumors. (whiterose.ac.uk)
  • While metastases re-epithelialize over time, we found that early metastases are remarkably mesenchymal, discarding the requirement for a mesenchymal-to-epithelial transition for early stages of metastatic growth. (whiterose.ac.uk)
  • Sarcomatoid conversion of clear cell renal cell carcinoma in relation to epithelial-to-mesenchymal transition. (lu.se)
  • Approximately 8% of clear cell renal cell carcinoma cases contain regions of radically different morphology, demonstrating a mesenchymal appearance histologically resembling sarcomas. (lu.se)
  • In the present study we investigate whether the sarcomatoid conversion of clear cell renal cell carcinoma could be interpreted as linked to the process of epithelial-mesenchymal transition. (lu.se)
  • Furthermore the sarcomatoid elements demonstrate an enhanced expression of epithelial-mesenchymal transition related mesenchymal markers as compared with the clear cell renal cell carcinoma counterparts. (lu.se)
  • We further selected a representative case, clinically demonstrating direct overgrowth of the sarcomatoid component into the liver and colon, for extended immunohistochemical characterization, resulting in a further set of positive and negative epithelial-mesenchymal transition markers as well as pronounced transforming growth factor β positivity, indicating that sarcomatoid clear cell renal cell carcinoma may be associated to epithelial-mesenchymal transition. (lu.se)
  • Corresponding changes in RNA levels for key epithelial-mesenchymal transition markers were also seen. (lu.se)
  • We therefore suggest that sarcomatoid clear cell renal cell carcinoma morphologically and immunohistochemically may represent a completed epithelial-mesenchymal transition and that transforming growth factor β1 could be an important driving force during the sarcomatoid transdifferentiation of clear cell renal cell carcinoma. (lu.se)
  • These microenvironments are classically defined as distinct cortex and medulla regions that each contain specialized subsets of stromal cells. (frontiersin.org)
  • While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)
  • In response to damage, epithelial wounds repair by a series of events that integrate epithelial responses with those of resident and infiltrating immune cells including neutrophils and monocytes/macrophages. (jci.org)
  • Our data showed that knockdown of HuR in pancreatic cancer cells significantly inhibited cell migration, invasion, and stem-like features. (ku.edu)
  • Restoration of HuR in HuR deleted (KO) pancreatic cancer cells enhanced migration and enriched CSC population. (ku.edu)
  • The data demonstrated that inhibition of HuR destabilized SNAI1 mRNA, and subsequently reduced Snail protein levels, resulting in the EMT and CSCs inhibition in pancreatic cancer cells. (ku.edu)
  • Skin and intestinal epithelial barriers play a pivotal role in protecting underlying tissues from harsh external environments. (jci.org)
  • While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
  • We introduce a class of Active Vertex Models (AVMs) for cell-resolution studies of the mechanics of confluent epithelial tissues consisting of tens of thousands of cells, with a level of detail inaccessible to similar methods. (cam.ac.uk)
  • The AVMs combine the Vertex Model for confluent epithelial tissues with active matter dynamics. (cam.ac.uk)
  • Tissues and Cells. (lu.se)
  • In this study, the protective effect of a fermented wheat germ extract (FWGE) against LPS-induced inflammation and oxidative stress in IPEC-J2 porcine intestinal epithelial cells was studied. (hindawi.com)
  • This electron micrograph highlights a darkly-stained glycocalyx rim of the brush border of the intestinal epithelium of the cat, stained en bloc with colloidal thorium. (ucsd.edu)
  • They are called transitional cells, renal (kidney) tubular cells, and squamous cells. (medlineplus.gov)
  • If there are squamous epithelial cells in your urine, it may mean your sample was contaminated. (medlineplus.gov)
  • The nuclei and the silver-stained nucleolar organizer region-associated proteins (AgNORs) in the normal, the dysplastic and the cancer cells of 38 cases of esophageal squamous cell carcinoma were measured by an image analysis system. (ac.be)
  • Chen Mao-huai & Wu Ming-yao , «Morphometric study on AgNORs of esophageal squamous cell carcinoma and paraneoplastic epithelial cells», Acta Stereologica [En ligne], Volume 11 (1992), Number 2 - Dec. 1992, 205-209 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=1952. (ac.be)
  • Materials and Methods: Mouse alveolar epithelial and alveolar macrophage cell lines and condensates collected at 3 worksites utilizing styrene -based resins were utilized for evaluations. (cdc.gov)
  • Specific markers on the surface of cTEC are Ly51 and CD 205 and even group of TECs expressing marker CD205 represent one of immature progenitors cells - cTEC committed progenitors. (wikipedia.org)
  • Double negative (DN) T cells, as a progenitors with CD44 and CD25 expression but lack of CD4 and CD8 coreceptor expression, are proliferated and differentiated to the double positive (DP) stages. (wikipedia.org)
  • This has been studied mainly in the small intestine, where different cell types such as enterocyte progenitors 4 , more differentiated secretory Bmi1 + cells 3 , or even fully differentiated Paneth cells can repopulate crypts upon injury. (nature.com)
  • This constant replacement of the epithelium is driven by cells located in the base of colonic crypts, which constantly divide and give rise to the various differentiated cell types. (nature.com)
  • We constructed stable knockdown (KD) cell lines for five epithelium -expressed miRNAs (miR-126, miR-141, miR-155, miR-210, and miR-1246) and assessed their response to infection with periodontal pathogens using microarray analysis , quantitative PCR (qPCR), enzyme-linked immunosorbent assay ( ELISA ), and Western blot assay. (bvsalud.org)
  • Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
  • We demonstrate that Rspo3 is essential for epithelial repair via induction of Wnt signaling in differentiated cells. (nature.com)
  • Thus, we find that endogenous Rspo3 signaling is a critical determinant of cellular fate within the crypt and stimulates rapid recruitment of differentiated cells for epithelial wound healing and crypt regeneration through induction of Wnt signaling. (nature.com)
  • These results demonstrate modulation of GLI-mediated transcription in both cancer and benign-derived epithelial cells by cyclopamine and sonic hedgehog, and further suggest that hedgehog signaling contributes to the survival of only the cancer epithelial cells. (rti.org)
  • Although the precise underlying mechanisms of AMD are not fully understood, numerous lines of evidence have indicated that endoplasmic reticulum (ER) stress contributes to the etiology of RPE cell damage and neovascularization formation ( 5 - 7 ). (spandidos-publications.com)
  • Dynamic remodeling of focal adhesion complexes and actin promotes interactions with the ECM that facilitate the epithelial sheet's migration. (jci.org)
  • Following initial epithelial cell migration, keratinocytes peripheral to the leading edge proliferate and mature to restore epithelial barrier homeostasis and function. (jci.org)
  • Vitamin A: The Importance in Health, Epithelial & Mucosa Lining, T-Cell Migration etc. (elisaact.com)
  • On this edition of "Ask Dr. J", Dr. Jaffe is asked to comment on Vitamin A's importance on health, epithelial and mucosa lining, T-cell migration and inhibition of IL6. (elisaact.com)
  • A platinum(II) complex HY1-Pt overcomes cisplatin-induced resistance and attenuates metastasis of epithelial ovarian cancer by cancer cell stemness inhibition. (physiciansweekly.com)
  • Recent researches reveal that cancer stem cells play important roles in the process of cisplatin-induced resistance and cancer cell metastasis. (physiciansweekly.com)
  • Patients diagnosed with sarcomatoid clear cell renal cell carcinoma face a considerably worse prognosis due to an increased propensity for metastasis. (lu.se)
  • The colonic epithelial turnover is driven by crypt-base stem cells that express the R-spondin receptor Lgr5. (nature.com)
  • The majority of epithelial cells are therefore short-lived, in contrast to a limited number of long-lived stem cells in the base of the crypts. (nature.com)
  • Lgr5 is a well-established marker of these basal stem cells in the gastrointestinal tract 1 and lineage tracing experiments have demonstrated that Lgr5 + cells give rise to clonal cell populations establishing entire colonic crypts 2 . (nature.com)
  • While Lgr5 + cells are long-lived and act as stem cells during homeostatic conditions, experimental models have demonstrated that Lgr5 + stem cells per se are not essential for maintaining epithelial integrity 3 . (nature.com)
  • Researchers with Sanford Burnham Prebys Medical Discovery Institute in La Jolla, California, were able to grow natural-looking hair that grows through the skin using human induced pluripotent stem cells (iPSCs). (pharmalive.com)
  • Pancreatic cancer is enriched with cancer stem cells (CSCs), which contribute to its high metastatic tendency and resistance to treatments. (ku.edu)
  • In the present study, we revealed that microRNA-30 (miR-30) members, especially miR-30b, were remarkably downregulated in triple-positive (CD24 + , CD44 + , EpCAM + ) pancreatic cancer stem cells (PCSCs). (jcancer.org)
  • Both of these types of cells can be defined and reciprocally distinguished by their expression of cytokines, chemokines, costimulatory molecules, and transcription factors, which have an effect on thymocyte development. (wikipedia.org)
  • When cells were treated with IL-1β and EGCG or hyperosmolarity and EGCG there was a dose-dependent reduction in release of these cytokines/chemokines, with significant inhibition observed at 3-30 µM. (molvis.org)
  • Our findings indicate that specific miRNAs regulate the expression of inflammatory cytokines in human gingival epithelial cells in response to microbial infection . (bvsalud.org)
  • Then TECs are divided into two phenotypically and functionally different groups that have distinct location, cytokeratin expression, surface markers, maturation factors, proteases and function in a T cell selection. (wikipedia.org)
  • Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. (lu.se)
  • Mean central thickness of non-stored limbal epithelial sheets was 23 ± 3 μm, and no substantial loss of cells was observed after storage. (datadryad.org)
  • The file presents data underlying the analyses for an article called "Serum-free and Xenobiotic-free Preservation of Cultured Human Limbal Epithelial Cells" in preparation for publication in PlosOne. (datadryad.org)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • The EasySep™ procedure involves labeling unwanted non-epithelial cells with biotinylated antibodies and magnetic particles. (stemcell.com)
  • Transfected HeLa cells were fixed in 3% paraformaldehyde, blocked with 0.2% Triton X-100, 1% FBS, 0.5% BSA in PBS with Ca[2+] and Mg[2+], followed by incubation in primary antibodies (anti-Myc mouse mAb, 9E10 (Covance) and anti-HA mouse mAb, HA.11 (Covance)) and secondary antibodies (Alexa 568 and Alexa 488) and DAPI (blue) to stain nuclei. (cellimagelibrary.org)
  • In this Review we focus on epithelial and innate immune cell interactions that mediate wound healing and restoration of tissue homeostasis in the skin and intestine. (jci.org)
  • We have shown that in the stomach, stem cell homeostasis is regulated by Wnt and Rspo secreted by stromal myofibroblasts 11 . (nature.com)
  • We demonstrate that while Rspo3 from myofibroblasts maintains colonic Lgr5 + cells during homeostasis, during injury its main function is not to maintain Lgr5 + cells but to interact with more differentiated cells that express Lgr4 but not Lgr5 and are able to regain expression of Wnt target genes and generate new crypts. (nature.com)
  • However, the function of RPL10 in human epithelial ovarian cancer (EOC) remains unknown. (jcancer.org)
  • Alveolar epithelial cells (AEC) have been implicated in pathological remodelling. (lu.se)
  • These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion. (harvard.edu)
  • These biphasic neoplasms are called sarcomatoid clear cell renal cell carcinoma. (lu.se)
  • TECs originate from non-hematopoietic cells that are characterized by negative expression of CD45 and positive expression of EpCAM. (wikipedia.org)
  • Expression of Cathepsin L and Cathepsin S is also typical for mTEC, because of participation of these proteases in the negative selection of T cells. (wikipedia.org)
  • Expression of Lgr5 is controlled by Wnt signaling, which plays a critical role for stem cell turnover in the gastrointestinal tract 2 . (nature.com)
  • Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. (nature.com)
  • In addition, mTEC screen thymocytes for their expression of high affinity αβ-TCRs, with negative selection limiting the release of these reactive T-cells into the peripheral pool ( 4 ). (frontiersin.org)
  • Cells from the bronchioloalveolar carcinoma cell line A549 were exposed to benzo(a)pyrene (B[a]P) for 24 weeks, and morphology, proliferative activity, and gene expression profiles were analyzed. (nih.gov)
  • Although no apparent morphologic changes were observed, the B[a]P-exposed A549 cells exhibited enhanced proliferative activity in 1% bovine serum that contained medium, and dramatic changes in expression levels were observed in a large number of genes. (nih.gov)
  • Using real-time, quantitative PCR, laser capture microdissection, and immunohistochemistry, distinctive patterns of expression of the hedgehog pathway members patched 1 (PTCH1), smoothened, GLI1, GLI2 and the 3 hedgehog ligands were identified for epithelial cells and stromal fibroblasts in benign breast and breast cancer. (rti.org)
  • Hedgehog-mediated transcription, as indicated by a reporter of GLI-dependent promoter activity and by expression of GLI1 transcripts, was reduced by the hedgehog pathway inhibitor cyclopamine in both MDA-MB-435 cancer epithelial cells and MCF10AT epithelial cells, a cell line derived from benign breast. (rti.org)
  • In addition, GSK2606414 treatment inhibited eIF2α phosphorylation and reduced CHOP and VEGF mRNA expression levels in RPE cells under TG‑induced ER stress. (spandidos-publications.com)
  • Biological studies indicated that HY1-Pt as a casein kinase 2 inhibitor could effectively overcomes cisplatin resistance through the signaling pathway of Wnt/β-catenin by inhibiting expression of the signature genes of cancer stemness cells in A2780/CDDP cells. (physiciansweekly.com)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells. (uni-muenchen.de)
  • In this study, we analysed the intracellular carbon metabolism of enteroinvasive Escherichia coli (EIEC HN280 and EIEC 4608-58) and Salmonella enterica Serovar Typhimurium (Stm 14028) replicating in epithelial colorectal adenocarcinoma cells (Caco-2). (uni-muenchen.de)
  • their evolution from distinct cell lineages. (lu.se)
  • The thymic microenvironment is established by TEC network filled with thymocytes (blood cell precursors of T cells) in different developing stages. (wikipedia.org)
  • Medullary epithelial cells (mTEC), in conjunction with dendritic cells, then take over control of developing thymocytes. (frontiersin.org)
  • In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)
  • Results showed that in cultured human-derived retinal pigment epithelial ARPE-19 cells , the extract of Chlorella zofingiensis and its nutritional ingredient astaxanthin exhibited significant inhibitory effects on the formation of endogenous N ε -carboxymethyllysine (CML), a key AGE representative, through the suppression of intracellular oxidative stress. (rsc.org)
  • This necessitates the investigation of past experimental approaches in their attempt to correlate oxidative stress and ocular diseases in humans in both the epithelial cells plus the capsule (LECs/capsule) and in the aqueous humor (AH). (molvis.org)
  • Since invasive pneumococcal strains adhere to, and translocate across, respiratory tract epithelial cells, adhesion is a prerequisite for establishing pneumococcal disease [ 4 ]. (ersjournals.com)
  • Stimulation of hedgehog signaling induces carcinogenesis or promotes cell survival in cancers of multiple organs. (rti.org)
  • The authors suggest that the morphometric study of AgNORs may be valuable in further studies on the change of AgNORs during the progression of the esophageal epithelial lesions towards cancer. (ac.be)
  • These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
  • HeLa cells expressing Myc-GnT1IP-L (Myc-tagged GlcNAcT-1 inhibitory protein, long transcript, membrane-anchored) (red) and GlcNAcT-1-HA (green) were treated with cycloheximide and brefeldin A to cause retrograde transport of Golgi enzymes into the ER. (cellimagelibrary.org)
  • Drugs diffuse across a cell membrane from a region of high concentration (eg, gastrointestinal fluids) to one of low concentration (eg, blood). (msdmanuals.com)
  • Because the cell membrane is lipoid, lipid-soluble drugs diffuse most rapidly. (msdmanuals.com)
  • A single exposure or multiple exposures of these cells to MNU was effective in inducing tumorigenic cells that produced palpable tumors as early as 6 weeks after transplantation. (eurekamag.com)
  • Rspo proteins are secreted and can stabilize the effects of Wnt ligands by preventing ubiquitination and turnover of the Wnt receptor frizzled 9 , thereby dictating the size of the Lgr5 + stem cell pool by regulating self-renewal of Lgr5 + cells 10 . (nature.com)
  • Angiotensin II Activates Extracellular Signal Regulated Kinases via Protein Kinase C and Epidermal Growth Factor Receptor in Breast Cancer Cells," Journal of Cellular Physiology, Vol. 196, No. 2, 2003, pp. 370-377. (scirp.org)
  • The non-stored epithelial sheets expressed a predominantly immature phenotype with ΔNp63α positivity of more than 3% in 9 of 13 cultures. (datadryad.org)
  • Title : Tissue cultures of KB epithelial cells for poliomyelitis virus tests Personal Author(s) : Luoto, Lauri;Pickens, Edgar G. (cdc.gov)
  • Mucus cells lining abnormal airways from patients with IPF expressed MUC5B and SOX2 but lacked SAM pointed domain containing ETS transcription factor (SPDEF) and forkhead box A3(FOXA3). (bmj.com)
  • Therefore a potential benefit pathologically to confirm the presence cells, which leads to abnormal differen- could be expected if we had a feasible or absence of gastritis and/or malignan- tiation of cells [3]. (who.int)
  • COX inhibitors have different selectivities for the two COX isoenzymes (COX 1 and COX 2) which vary between purified enzyme and intact cell preparations. (ersjournals.com)
  • Upon Lgr5 + cell depletion, these cells are recruited to contribute to crypt regeneration. (nature.com)
  • In addition, RPE cells proliferate and secrete various proangiogenic factors, including vascular endothelial growth factor (VEGF), which serves an important role in AMD-associated CNV ( 4 ). (spandidos-publications.com)
  • Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. (lu.se)
  • Combined treatment of FWGE and 5-fluorouracil or dacarbazine resulted in a synergistic effect in the HCR-25 human cancer cell line and B16 murine melanoma cell line [ 6 ]. (hindawi.com)
  • The current results indicated that B[a]P seems to induce EMT in lung cancer cells, and it also may drive disease progression in patients with lung cancer. (nih.gov)
  • together with protein concentration) and lens epithelial cells plus capsule (LECs/capsule) in patients with cataract (CAT), and also suffering from pseudoexfoliation syndrome (PEX), primary open-angle glaucoma (POAG) and pseudoexfoliation glaucoma (PXG). (molvis.org)
  • Graph of the correlation between serum 17-HPG concentration and percentage of cornified preputial epithelial cells in 21 castrated ferrets. (avma.org)
  • There are three types of epithelial cells that line the urinary tract. (medlineplus.gov)
  • The oil immersion lens (x1000) was switched and between 10 and 20 representative fields was examined to observe cell morphology and gram reaction. (cdc.gov)